BIN1 Antibodies

Myc box-dependent-interacting protein 1 (BIN1)

Is a key player in the control of plasma membrane curvature, membrane shaping and membrane remodeling. Required in muscle cells for the formation of T-tubules, tubular invaginations of the plasma membrane that function in depolarization-contraction coupling (PubMed:24755653).

Is a negative regulator of endocytosis (By similarity). Is also involved in the regulation of intracellular vesicles sorting, modulation of BACE1 trafficking and the control of amyloid-beta production (PubMed:27179792).

Gene Information

Human
Gene Name:	BIN1
Uniprot:	O00499
Entrez:	274

Family

Belongs to:
No superfamily

Alternative Names

AMPH2; Amphiphysin II; Amphiphysin-like protein; AMPHLDKFZp547F068; box dependant MYC interacting protein 1; bridging integrator 1Box-dependent myc-interacting protein 1; MGC10367; myc box-dependent-interacting protein 1; SH3P9

Molecular Weight

Mass (kDA):
64.699 kDA

Genomic Context

Human
Location:	2q14.3
Sequence:	2; NC_000002.12 (127048023..127107154, complement)

Tissue Specificity

Ubiquitous. Highest expression in the brain and muscle (PubMed:9182667). Expressed in oligodendrocytes (PubMed:27488240). Isoform IIA is expressed only in the brain, where it is detected in the gray matter, but not in the white matter (PubMed:27488240). Isoform BIN1 is widely expressed with highest expression in skeletal muscle.

Subcellular Localizations

[Isoform BIN1]: Nucleus. Cytoplasm. Endosome. Cell membrane, sarcolemma, T-tubule.; [Isoform IIA]: Cytoplasm.

Diseases

• Neoplasms
• Malignant Neoplasms
• Alzheimer's Disease
• Cell Transformation, Neoplastic
• Myopathy
• Congenital Structural Myopathy
• Malignant Paraganglionic Neoplasm
• Malignant Neoplasm Of Breast
• Weakness
• Mammary Neoplasms
• Carcinoma
• Congenital Myopathy (disorder)
• Dementia

• Malignant Neoplasm Of Prostate
• Neoplasm Metastasis
• Metaplastic Cell Transformation
• Liver Carcinoma
• Neuroblastoma
• Muscle Weakness
• Tumor Progression

Interacting Proteins

• BIN2
• DLGAP4
• FBP1
• MTM1
• MYC

• SNX4
• XRCC4

Pathways

• Endocytosis
• Localization
• Pathogenesis
• Cell Death
• Cell Proliferation
• Cell Cycle
• Dna Repair
• Programmed Cell Death
• Aging
• Methylation
• Cell Growth
• Dna Methylation
• Transport

• Clathrin-mediated Endocytosis
• Cytokinesis
• Cell Motility
• Cell Differentiation
• Cell-cell Adhesion
• Oncogenesis
• Chromatin Remodeling

PTMs

• Phosphorylation
• Methylation

• Cleavage
• Biotinylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/BIN1

Boster Bio Anti-BIN1 Marker

This article will examine the Boster Bio Anti-BIN1 Marker (and its applications). We'll also look at the history of Steven Boster who created this marker. We hope you are able to use this information in your decision-making process. We'll also provide tips on how to best use the marker. The most important thing to take into consideration when choosing an anti-BIN1 antibody is the ability to react.

Boster Bio Anti-BIN1 Marker

The Boster Bio Anti-BIN1 Monoclonal Antibody catalog number M01551 reacted to Human. This monoclonal antibody recognizes BIN1 which is a novel gene product found on chromosome 2. It is critical for normal muscle function and the positioning of nuclei. Boster Bio validates all of its antibodies prior to making them available for sale.

Boster Bio produces more than 12,000 antibodies, many of which are validated for use in IHC, WB, Flow, and ELISA. Boster Bio's antibody is subject to rigorous and qualitative testing against 250 untransfected cell lines and tissues. This guarantees a high level of affinity, specificity, and sensitivity. Boster Bio offers a wide selection of applications that can be used for a range of research methods. It also provides technical support for its antibodies.

Applications

The Boster Bio Anti-BIN1/Amphiphysin Ii Monoclonal Antibody, catalog number M01551 reacts with Human. BIN1 is a novel human gene product. It is located on the 22q14 chromosome. BIN1 mutations can lead to chronic muscle pain, also known as centronuclearmyopathy. Normal muscle function and the positioning of nuclei are influenced by BIN1 and DNM2. Boster Bio antibodies have been tested for specificity and accuracy.

The BIN1 marker is a powerful method for detecting tumor-related protein levels. Tumor cells can be identified by the BIN1 gene when they are when there is a human leukemia present. These tumors have abnormal BIN1 expression, which indicates they are infiltrating. The BIN1 marker is also useful in detecting malignant tumors and identifying cancer cells. The BIN1 gene plays a role in the progression of cancer, as well as the development of the immune systems.

Quality

The BIN1 marker was used in the present study to detect Tau proteins. The FRET signals were detected on 3/10 samples, indicating that BIN1 was present in Tau-containing EVs. Additionally, BIN1 was associated with seeding capable EVs. The BIN1 marker can also be used to measure Tau levels of Tau protein in cells. This method can also be used to determine the relationship of DNM2 and BIN1, a protein involved with normal muscle function, and nucleus positioning.

BIN1 immunoprecipitates revealed two different forms that were compatible with isoforms 6-9 However, no Tau antibodies were identified. The BIN1 immunopurified EVs showed a similarity to exosomes, as measured by nanoparticle track analysis. Although the results of this study are positive however, there are some limitations. This is especially true regarding the quality of the BIN1 marker in Boster Bio.

Steven Boster's story

Steven Boster invents his first product in 1993. His name means "he who transforms science in the toilet," and he was named that ever since. Boster was the first catalog antibody from China company and later created IHC products and primary antibodies. Boster had invented the first high-sensitivity ELISA kits as well as a unique ELISA platform PicoKine(tm) towards the end of the 90s.