BCAT1 Antibodies

Branched-chain-amino-acid aminotransferase, cytosolic (BCAT1)

Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine. .

Gene Information

Human
Gene Name:	BCAT1
Uniprot:	P54687
Entrez:	586

Family

Belongs to:
class-IV pyridoxal-phosphate-dependent aminotransferase family

Alternative Names

BCAT(c); BCAT1; BCAT-1; BCATC; BCT1; BCT1PNAS121; branched chain amino-acid transaminase 1, cytosolic; branched chain aminotransferase 1, cytosolic; branched-chain-amino-acid aminotransferase, cytosolic; DKFZp686E12175; EC 2.6.1.42; ECA39; MECA39; placental protein 18; PNAS121; PP18; Protein ECA39

Molecular Weight

Mass (kDA):
42.966 kDA

Genomic Context

Human
Location:	12p12.1
Sequence:	12; NC_000012.12 (24810024..24949101, complement)

Tissue Specificity

During embryogenesis, expressed in the brain and kidney. Overexpressed in MYC-induced tumors such as Burkitt's lymphoma.

Subcellular Localizations

Cytoplasm.

Diseases

• Neoplasms
• Malignant Neoplasms
• Cell Transformation, Neoplastic
• Tissue Adhesions
• Neoplasm Metastasis
• Burkitt Lymphoma
• Carcinoma
• Lymphoma
• Ovarian Neoplasm
• Diabetes Mellitus
• Nervousness
• Adenoma
• Malnutrition

• Malignant Neoplasm Of Breast
• Hepatitis C, Chronic
• Seminoma
• Childhood Ependymoma
• Ulcer
• Neoplasms, Glandular And Epithelial
• Varicose Ulcer

Pathways

• Cell Adhesion
• Cell Proliferation
• Cell Cycle
• Oncogenesis
• Transport
• Fruit Development
• Localization
• Endocrine Signaling
• Coagulation
• Fatty Acid Transport
• Cell Death

• T Cell Proliferation
• Regulation Of Cell Proliferation
• Cell Growth
• Wound Healing
• Pathogenesis
• Rna Interference

PTMs

• Phosphorylation

• Amination

Research Areas

• Cell Cycle and Replication
• Core ESC-Like Genes
• Stem Cell Markers

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/BCAT1

Boster Bio - Best Uses Of The BCAT1 Marker

This article will outline some of the Boster Bio benefits: best uses, druggability and IHC. Other benefits may be of interest to you, such as the ability for scientists around the world to submit their results for species and applications. All Boster scientists welcome to submit results and receive product credit for their work. All scientists worldwide can benefit from the information in this article.

IHC

The BCAT1 gene encodes a transaminase called Branched-chain amino-transferase. It is responsible to catalyze the reversible transamination a-keto acids, which are essential for cell proliferation. When the BCAT1 gene is mutated, the resulting protein is hypervalinoemic and hyperleucine-isoleucine.

IHC studies showed that BCAT1 inhibits IRG1 transcription and itaconate levels within macrophages stimulated by LPS. BCAT1 is also involved with glycolytic metabolism. Gabapentin could also target BCAT1 as a secondary target. It binds with the a2d subunits voltage-activated Calcium channels, but has no antiinflammatory effects.

Boster biosystem's BCAT1 gene is expressed in high amounts in many different tissues. It is regulated at multiple levels. These include the TCA and oxidative Phosphorrylation. Moreover, BCAT enzymes may contribute to metabolic reprogramming in eukaryotic cells. These results are encouraging. BCAT1's usefulness in RNAi experiments is another area. The present study involved transfection with BCAT1 siRNA on hMDMs and stimulation with LPS.

Boster bio's BCAT1 marker is used to stain tissue sections with immunohistochemical staining. This involves the use of a rabbit anti–BCAT1 monoclonal anti antibody and a mice anti–C–Myc monoclonal anti antibody. To ensure binding, the antibodies should be incubated at 37°C (for one hour). A blemish is a tumor tissue that has not been detected by the BCAT1 marker.

ELISA

ELISA (enzyme-linked immunosorbent test) is an enzyme. The basic principle behind ELISA is similar to other immunoassay techniques: A sample is exposed and binds to an antigen. The antigen then binds to a particular reporter enzyme. The ELISA utilizes high-affinity antibodies to increase sensitivity. Boster Bio has perfected the process and its products have been trusted by scientists all over the world.

ELISA is a simple method that detects multiple analytes simultaneously using only one antibody. This method has a low cost advantage. This kit costs only 25 microliters of DNA and is 30% cheaper than other multiplex assays. A second advantage is the ultrasensitive DNA amplification process that increases detection sensitivity.

RNAi can also be used to detect BCAT1 protein from a variety of samples. A study conducted by Boster Bio has demonstrated the accuracy of the method, and the ability to detect BCAT1 in a variety of cell types. BCAT1 can be used as an indicator gene. An ELISA can also identify BCAT1 proteins in serum, RNAi and mRNA samples.

The ELISA-plate can be used on a variety surface types and is designed for optimal results. Thermo Scientific's ELISA plates have a variety of surface treatments. All surface treatments are designed to ensure reproducibility as well as lot-to–lot reliability. They also come with many blockers. If you're creating an ELISA for research purposes, be sure to choose the right blocking agent. For example, an antigen antibody with low affinity may give a lower signal level than one with a higher concentration.

Druggability

In immuno-oncology studies have examined the druggability for BCAT1 marker. Temporal inhibition of BCAT1 results a high number of CD8+ T cell populations with increased proliferative capacity and cytotoxic potential. This marker is a potential druggable target in immuno-oncology because of its high recombination rate. This finding requires further investigation.

Applications

The potential applications of BCAT1 in cancer research are evident. Numerous studies have shown an association between BCAT1 levels and the progression of breast cancer in triple-negative breast (TNBC) patients. BCAT1 levels were higher in tamoxifen -resistant TNBC than non-resistant TNBC. Also, lower levels of BCAT1 expression were associated to poor prognosis. To confirm the association between BCAT1 expression in TNBC and tumour progression, larger clinical trials will be needed.

In vitro testing showed that the CXXC mutation in BCAT1 caused a decrease in H2O2 levels. This suggests that BCAT1 could play an antioxidant role. BCAT1 modulates intracellular ROS levels and may therefore have antioxidant properties. Further, this motif was found to increase survival of AML cells. These findings have important clinical implications. There is a strong connection between BCAT1 (the ability of tumor cells tolerate serum-deprivation) and BCAT1.

To validate the BCAT1 gene-expression pattern, RNA was extracted from puromycin-resistant U937 cell clones and synthesised into cDNA using PCR Biosystems' cDNA synthesis kit. The RNA samples were analysed for BCAT1 expression and beta-2-microglobulin(B2M), using qPCR primers. qPCR was carried out on a Roche 480LightCycler. 95 degC was used for 10 s, and 63 for 10 s to determine BCAT1 and GAPDH expression.

Product range

The BCAT1 protein encodes an indicator that cancer is possible. The BCAT1 protein is overexpressed in glioblastoma tumor cells. In addition to this, BCAT1 cooperates with the enzyme IDH to degrade branched-chain amino acids, which serve as "food" for cancer cells. Furthermore, branched -chain amino acids play an important part in metabolic diseases such diabetes.