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- Table of Contents
Facts about RNA demethylase ALKBH5.
Requires molecular oxygen, alpha-ketoglutarate and iron (PubMed:21264265, PubMed:23177736, PubMed:24778178, PubMed:24616105, PubMed:24489119). Demethylation of m6A mRNA affects mRNA processing and export (PubMed:23177736).
Human | |
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Gene Name: | ALKBH5 |
Uniprot: | Q6P6C2 |
Entrez: | 54890 |
Belongs to: |
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alkB family |
ABH5OFOXD; alkB, alkylation repair homolog 5 (E. coli); Alkylated DNA repair protein alkB homolog 5; EC 1.14.11.-; FLJ20308; OFOXD1; oxoglutarate and iron-dependent oxygenase domain containing; probable alpha-ketoglutarate-dependent dioxygenase ABH5
Mass (kDA):
44.256 kDA
Human | |
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Location: | 17p11.2|17p11.2 |
Sequence: | 17; NC_000017.11 (18183828..18209954) |
Widely expressed, with highest expression in lung, followed by testis, pancreas, spleen and ovary.
Nucleus speckle.
The ALKBH5 gene encodes an enzyme that demethylates the mRNA. This enzyme is necessary to remove m(6)A, which is the most common modification that is found in mRNAs. Boster validates all antibodies on known positive and negative samples. If your results are positive, you'll receive with credits for the product. These credits can be used to purchase any Boster Bio product.
The crystal structure of FTO/m6dA was described as PDB ID 5ZMD. FTO catalyzes oxidation of the m6A m6A nucleobase and forms hm6A. The m6dA nucleobase makes hydrogen bonds with N6 and N7 endocyclic nitrogen . It also adds these residues to partial positive charges. The positive charge is lowered to the molecular orbital with the greatest amount of occupied space as is the case for the N6 lone couple.
Despite the massive loop however, not all residues that compose the active site are conserved. The modelled structure showed conserved residues for recognition and demethylation. These structural insights will be helpful in the development of functional probes and inhibitors. The molecule is a DNA binding protein with a dimerization region and the N-terminal loop is huge. The process of discovering drugs is based on molecular structure.
ALKBH5 mutants exhibit a remarkable increase in thermal stability when incubated with NOG, a general inhibitor. ALKBH5 also has a lower affinity for f6A. Additionally the mutant ALKBH5 has a higher affinity for Mn(II).
ALKBH5 differs from FTO in that it has a distinct function for demethylation of m6A. This is due to the interaction between R130 and K132 and Y139 with the m6A intermediate. Demethylation of m6A is attainable only through the use of ALKBH5. This is why FTO is a good candidate for a marker for the m6a gene. This gene is associated with many cancer risk factors, including Melanoma.
The ALKBH5 gene is widely expressed in lung adenocarcinoma cells especially those experiencing intermittent hypoxia. Hypoxia-induced upregulation of this gene promoted lung adenocarcinoma cell proliferation and invasion. ALKBH5 inhibited the expression of FOXM1's expression of mRNA. Furthermore, the protein's expression encouraged the development of tumors in living. ALKBH5 also inhibited the activity of YTHDFs-mediated YAP.
The ALKBH5 gene is known to negatively regulate osteogenic differentiation of MSCs. Functional studies have shown that this gene inhibits osteogenic differentiation through the regulation of PRMT6. It also regulates the PI3K/AKT signaling pathway, which hinders osteogenesis. ALKBH5 was also knocked down in MSCs, which resulted in increased bone mass in mice.
The transcriptional regulator of eNOS is the ALKBH5 gene. eNOS is required by ECs to produce nitric oxygen. Growth hormone signaling and angiogenesis are controlled through activation of the AKT/eNOS Achilles. Inhibition of eNOS by ALKBH5 inhibits the phosphorylation of eNOS.
The ALKBH5 gene is among nine genes belonging to the AlkB family. It is a ferrous iron-dependent, 2-oxoglutarate-dependent gene that catalyzes demethylation of m6A RNA. Feng et. and. also identified the crystal structure of ALKBH5 in humans and Zebrafish.
To better understand the complexity of this disease, it is essential to ensure that the ALKBH5 gene marker is confirmed in pancreatic carcinoma. Recent research has shown that ALKBH5 is linked to the methylation process of related genes and mRNA. Epigenetic modifications could be a factor in the development of pancreatic cancer and play an important role in cancer development. This marker is a potential candidate for application in the clinical setting and may help in developing new therapeutic and diagnostic strategies.
Prognosis for PDAC is poor due to low levels of ALKBH5 expression in PDAC. Furthermore, the downregulation of this gene correlates with poor prognosis. These findings were confirmed by gene expression studies. PDAC is only diagnosed by the ALKBH5 marker. Validation of ALKBH5 is a way to identify patients who are at risk for the disease.
In the TCGA and ICGC cohorts, the expression of ALKBH5 gene had a high prognostic value. The AUC value of the ALKBH5 gene was significantly associated with three-year survival. The results of the ROC curve suggested that the ALKBH5 marker may predict the outcome of pancreatic cancer patients. In the TCGA and ICGC cohorts, ALKBH5 gene expression showed the highest correlation with overall survival.
To validate the ALKBH5 marker for pancreatic cancer, a human cell line, MIA PaCa-2, was used. It was grown with 10% FBS in DMEM. It was transduced using the lentiviruses and then selected for analysis by a limited dilution assay. Additionally, the m6A-IP library had high read density. Further, exon junctions inside MM cells were identified by a read number.
The ALKBH5 marker can be used to identify cancer stem cells. It is responsible for the differentiation of breast cancer stem cells, enlargement in the tumor's microenvironment and proliferation of tumor-initiating cells. It is involved in tumorigenesis by catalyzing the demethylation NANOG mRNA. The ALKBH5 gene could be a candidate for treatment with tumor-suppressors.
The gene ALKBH5 regulates the expression of EGFR. The expression of ALKBH5 in pancreatic cancer was linked with a higher probability of overall survival. Multicohort studies revealed that the expression of ALKBH5 was positively associated with survival. It was also considered an independent predictor of the cancer. Although the function of ALKBH5 is not fully understood but there are some promising clinical applications for this gene marker.
ALKBH5 can be present in all cell types and tissues. However it is not present in all cells. ALKBH5 expression is linked to ALP activity in 15 people. The results are presented as means and standard deviations (SDs) for each group. The ALKBH5 gene is part of the PRMT6 gene. This gene is an downstream target of ALKBH5.
This gene is also involved in osteogenesis. ALKBH5 is vital for osteogenic differentiation in MSCs. It blocks the PI3K/AKT pathway. Further research is needed to discover the genes that are controlled by the ALKBH5/PRMT6 axis. These results suggest that small-molecule inhibitors of the ALKBH5 axis could be developed in MSCs. These inhibitors could be beneficial to the development of MSC-based techniques for tissue engineering.
Scientists who are interested in using Boster Bio ALKBH5 ELISI Kits can submit their findings for specific species, samples, and applications. Boster scientists also get credits for their work. These kits are suitable for scientists worldwide. This article will discuss the ALKBH5 ELISA Kit's functions. Find out more. This article originally appeared on the website of Scientific America.
The anti-RNA demethylase antibody is boster bio reagent, which reacts with Mouse and Human samples. This reagent can be used for IHC or WB applications. It has a picogram-level sensitivity. Boster Bio Anti-RNA demethylase ALKBH5 antibody has been tested for use in immunofluorescence and IHC applications. Technical support is available from Sanbio for all Boster Bio Reagents.
The ALKBH5 genewhich is a post-transcriptional regulator for multiple genes that have distinct roles in human cancers, is a prime instance. There are genes that regulate the cell cycle, apoptosis DNA repair, metabolism, and autophagy. The exact role of ALKBH5 will depend on the type of cancer and can be contradictory. In certain instances however, it can be beneficial to cancer cells. In other instances it could be harmful.
The ALKBH5 marker is an protein that binds to RNA and demethylates a sequence that was deemed non-conventional. This results in lower yields. The ALKBH5 marker is able for a high-quality expression test. This RNA-based test can be obtained from many sources. The cost of the ALKBH5 marker can differ depending on the source of the DNA sample.
PMID: 17979886 by Tsujikawa K., et al. Expression and sub-cellular localization of human ABH family molecules.
PMID: 21264265 by Thalhammer A., et al. Human AlkB homologue 5 is a nuclear 2-oxoglutarate dependent oxygenase and a direct target of hypoxia-inducible factor 1alpha (HIF- 1alpha).
*More publications can be found for each product on its corresponding product page