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- Table of Contents
Facts about Short/branched chain specific acyl-CoA dehydrogenase, mitochondrial.
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Human | |
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Gene Name: | ACADSB |
Uniprot: | P45954 |
Entrez: | 36 |
Belongs to: |
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acyl-CoA dehydrogenase family |
2-MEBCAD; 2-methylbutyryl-coenzyme A dehydrogenase; ACAD7,2-methylbutyryl-CoA dehydrogenase; acyl-CoA dehydrogenase, short/branched chain; acyl-Coenzyme A dehydrogenase, short/branched chain; EC 1.3.99,2-methyl branched chain acyl-CoA dehydrogenase; EC 1.3.99.-; SBCADshort/branched chain specific acyl-CoA dehydrogenase, mitochondrial
Mass (kDA):
47.485 kDA
Human | |
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Location: | 10q26.13 |
Sequence: | 10; NC_000010.11 (123009006..123058317) |
Ubiquitous.
Mitochondrion matrix.
In this article we will examine the ACADSB genes, ACADSB proteins, antibodies, and ELISA platforms. Learn more about ACADSB if you are interested in testing for it in your research. We will also discuss Boster Bio’s custom-services, such as BeNeLux Delivery. You can also download their customer guide.
The metabolism is controlled by the ACADSB, also known to be adenosine A2a. This is vital for the growth of muscles. It is a protein with multiple roles, including cell growth control, cell signaling, and regulation metabolism. Although there are many different mutations in the gene known, a recent study identified a new variant that causes ataxia.
The ACADSB gene contains instructions for the production short/branched-chain acyl-CoA deshydrogenase (or SBCAD). This enzyme plays a key role in protein processing. When you digest protein it is broken down to amino acids, which the body can then use to create energy. SBCAD can be found in mitochondria, which is the cells that transform food into energy.
The ACADSB gene not only influences transcription levels but also influences other genes. The ACADSB gene was found to be associated with 4,118 biological entities in this study. These associations can be useful in determining the exact function or cause of a specific disease or tissue. For its potential role in cancer or other diseases, it is recommended that ACADSB is studied in human tissues and cell culture.
ACADSB, a member the acylCoA Dehydrogenase Family, is an important gene involved in milk fat synthesis. ACADSB has been identified in numerous studies as an important candidate gene for milk-fat synthesis. A bMEC knockout of this gene is a useful experimental tool for further research. It may also reveal the function of ACADSB for fatty acid synthesis.
The ACADSB gene may be involved in lipid metabolism in the aging process. Interestingly enough, ACADSB-knockout mice are able produce milk in vitro and from mammary tissue. This gene could help researchers understand how this gene affects milk lipid metabolism. The gene can be altered with the use of CRISPR-Cas9 technology.
The ACADSB protein is a component in the adenosine Monophosphate pathway. The enzyme is necessary for the breakdown fatty acids in human bodies. Its mRNA level is upregulated in bMECs but downregulated in ACADSB/ mice. The ACADSB enzyme is capable of metabolizing Adenosine Monophosphate into acetylCoA (or isobutyrylCoA)
ACADSB is a member the acyl-CoA family and plays a crucial role in fatty acid metabolic. It has recently been proposed as a candidate gene involved in milk fat synthesis. The gene's function was verified using a CRISPR/Cas9 system in bMECs, which are derived from adipose tissue of milk cows. It was also sequenced in the CRISPR/Cas9 method and analyzed for lipid metabolism.
ACADSB is a key regulator of lipid metabolism at bMECs. Studies have shown ACADSB regulates a number of other genes that are involved in this pathway. ACOX2 & ACADL are just two of the genes that are affected by ACADSB. ACADSB plays a crucial role in lipid metabolism. This has been confirmed by studies. Moreover, ACADSB influences synthesis of the milk fat component in vivo.
Adaptive cloning is a common method for testing the efficacy and safety of a new molecule. It is possible to clone ACADSB in a vector containing the cloning site of ClaI/BamHI. Once the enzyme is cloned to pCMV3, a vector containing a particular gene is ready for use.
ACADSB has no approved dietary supplement. Boster Bio ACADSB enzyme is one of the best-known products for this purpose. Researchers have found more than 100 gene variants that can be linked to ACADSB. They are now using them in their own research. They plan to use this gene in their research to better understand the mechanisms of lipid metabolism. And as an added bonus, they have discovered that ACADSB affects the expression of other genes involved in cell metabolism.
Anti-ACADSB antibody helps to detect ACADSB within a cell. The enzyme is detected in immunocytochemical sections of Mcf-7 cells. AR0022 was used to retrieve the enzyme antigen. After the cells had been blocked with 10% goat serum, they were incubated with 5OEog/mL rabbit anti-ACADSB antibody and DyLight!AE488 Conjugated Goat Anti-Rabbit IgG (BA1127) antibody for 30 minutes. Finally, the sections of the cells were counterstained with DAPI.
Picoband(tm), part of the Picoband(tm), includes the Boster Bio ACADSB antibodies. These antibodies are specifically made to target ACADSB protein. They react with human, mouse, and rat samples. The immunoreactive antibody is suitable to be used in Immunofluorescence or Flow Cytometry. To get the best results, dilute the antibody to the right concentration for each assay.
ACADSB/ bMECs express ACADSB gene in a less than wild-type bMECs. This is a consequence of decreased TG and CHOL content, respectively. The data in this graph are expressed as mean + SEM. These data don't fully account for ACADSB dependent expression in bMECs.
To determine the cellular mRNA concentration, RNAi-based knockdown of ACADSB and overexpression were used. RNAi-transfected cells had significantly decreased ACADSB mRNA expression compared to overexpression-transfected cells. Further experiments are necessary to confirm the quality the resulting antibodies. Boster Bio antibodies are made to recognize and neutralize ACADSB, which is an important component in many cellular structures.
The expression levels of the ACADSB gene were assessed in mammary tissues taken from dairy cattle with different milkfat contents. The ACADSB gene was found to be involved in regulating fatty acid and lipid metabolism in bMECs. Moreover, ACADSB/antibodies reduced TG synthesis by bMECs compared with wild-type controls. These results confirm the reliability of Boster Bio's ACADSB antibodies.
The results of the RNAi and overexpression experiments have been reported. Boster Bio ACADSB antibody revealed that ACADSB/ cells had a significantly lower lipid level than WT cells. The experiments reveal that ACADSB cells are a valuable tool in evaluating lipid related genes in bMECs. After these cells were isolated, they were treated using RNAi or overexpression vectors. They were harvested 24 hours later. Total RNA was extracted using a PREP RNA mini kit and cDNA was synthesized using the RT cDNA synthesis kit. qRT-PCR was conducted after the RNAi treatments. The reaction was done using SYBR (r) Premix Ex-taqTM and ACADSB -F
Contact the company to inquire about purchasing the ACADSB antibody. The company also offers customized services, and BeNeLux shipping. Sanbio's website has more information on Boster Bio ACADSB antibodies. They are always happy to assist. It is worth noting that they are a trusted supplier to biological research. Sanbio is available for questions regarding the ACADSB antibody.
ACADSB, a lipid-associated gene, regulates fatty acids and lipid metabolism. Its knockout mutants exhibit a reduced lipid content when compared to control cells. These results show the importance of this gene in lipid metabolism. To learn more about ACADSB, we will explore the functions of ACADSB in this platform. In the next paragraph we will look at some of the applications that the ACADSB ELISA platform can be used for.
ACADSB expression is associated with the pathological characteristics and tumors. ACADSB expression is associated with tumor grade, pathological stage T, and N/M stages. Two experienced pathologists validated ELISA's platform. The data was also used for the generation ROC curves. To evaluate the diagnostic performance of ACADSB, we also calculated area under the curve.
ACADSB ELISA panels use sandwich enzyme-linked immune-sorbent technology. The antibody is first coated on a plate. Then, the biotin-conjugated reaction is used. HRP enzymatic reaction is quantified using TMB substrate. The colour of the plate changes from blue to yellow after adding TMB substrate. This indicates the amount of ACADSB that is bound to it.
ACADSB can be found in a wide variety of cells, including mammary tumors and glioma. The protein has been linked with several cancers. It may also be used to diagnose them. ACADSB can promote tumor growth if it is reduced. It can also inhibit the breakdown of FA and BCAAs and thus impair ferroptosis. These data prove the value of ACADSB as a prognosticator for a cancer.
The ACADSB ELISA platform measures ACADSB levels in a variety of biological fluids. Its detection limits can be determined using a standard curve or duplicate readings. Its validity is for six months. Although ACADSB exists in many cell types in humans, it is more prevalent in the mitochondrial cells. In some instances, it is present in the brain, but its expression is not as high as expected.
The ACADSB genome contains instructions for short/branched acyl CoA dehydrogenase. This enzyme plays an important role in protein metabolism. It breaks down proteins to amino acids, which are then used by our bodies for energy. SBCAD is located in mitochondria, which are cells responsible for converting food into energy. ACADSB, which is found in the brain can regulate fatty acids metabolism.
PMID: 7698750 by Rozen R., et al. Isolation and expression of a cDNA encoding the precursor for a novel member (ACADSB) of the acyl-CoA dehydrogenase gene family.
PMID: 10832746 by Gibson K.M., et al. 2-methylbutyryl-coenzyme A dehydrogenase deficiency: a new inborn error of L-isoleucine metabolism.