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Product Info Summary
| SKU: | LS000274 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of bridging integrator 1 (BIN1), transcript variant 1 |
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Product info
Product Name
BIN1 (NM_139343) Human Over-expression Lysate
SKU/Catalog Number
LS000274
Size
100 µg
Description
Transient overexpression lysate of bridging integrator 1 (BIN1), transcript variant 1
Storage & Handling
The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2xSDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Cite This Product
BIN1 (NM_139343) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS000274)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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BIN1 Human Over-expression Lysates NM_004305.
Western blot validation of overexpression lysate (LS000274) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with BIN1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
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BIN1 Human Over-expression Lysates NM_139343.
Western blot validation of overexpression lysate (LS000274) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with BIN1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Click image to see more details
BIN1 Human Over-expression Lysates NM_139344.
Western blot validation of overexpression lysate (LS000274) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with BIN1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Click image to see more details
BIN1 Human Over-expression Lysates NM_139346.
Western blot validation of overexpression lysate (LS000274) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with BIN1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Click image to see more details
BIN1 Human Over-expression Lysates NM_139347.
Western blot validation of overexpression lysate (LS000274) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with BIN1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Click image to see more details
BIN1 Human Over-expression Lysates NM_139348.
Western blot validation of overexpression lysate (LS000274) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with BIN1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Click image to see more details
BIN1 Human Over-expression Lysates NM_139349.
Western blot validation of overexpression lysate (LS000274) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with BIN1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Click image to see more details
BIN1 Human Over-expression Lysates NM_139350.
Western blot validation of overexpression lysate (LS000274) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with BIN1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for BIN1 (Source: Uniprot.org, NCBI)
Gene Name
BIN1
Full Name
Myc box-dependent-interacting protein 1
Weight
64.699kDa
Alternative Names
AMPH2; Amphiphysin II; Amphiphysin-like protein; AMPHLDKFZp547F068; box dependant MYC interacting protein 1; bridging integrator 1Box-dependent myc-interacting protein 1; MGC10367; myc box-dependent-interacting protein 1; SH3P9 BIN1 AMPH2, AMPHL, CNM2, SH3P9 bridging integrator 1 myc box-dependent-interacting protein 1|amphiphysin II|amphiphysin-like protein|box dependant MYC interacting protein 1|box-dependent myc-interacting protein 1
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on BIN1, check out the BIN1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BIN1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For BIN1 (NM_139343) Human Over-expression Lysate (LS000274)
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