ZW10 interactor Antibodies

ZW10 interactor (ZWINT)

Part of the MIS12 complex, which is required for kinetochore formation and spindle checkpoint activity. Required to target ZW10 to the kinetochore in prometaphase.

.

Gene Information

Human
Gene Name:	ZWINT
Uniprot:	O95229
Entrez:	11130

Family

Belongs to:
No superfamily

Alternative Names

C20orf164; chromosome 20 open reading frame 164; dJ337O18.7; zinc finger SWIM domain-containing protein 3; zinc finger, SWIM domain containing 3; zinc finger, SWIM-type containing 3

Molecular Weight

Mass (kDA):
31.293 kDA

Genomic Context

Human
Location:	10q21.1
Sequence:	10; NC_000010.11 (56357227..56361273, complement)

Subcellular Localizations

Nucleus. Chromosome, centromere, kinetochore. Localizes to kinetochores from late prophase to anaphase.

Diseases

• Malignant Neoplasms
• Neoplasms
• Malignant Neoplasm Of Breast
• Pain
• Stenosis
• Aneuploidy
• Mammary Neoplasms
• Cell Transformation, Neoplastic
• Neuralgia
• Leukemia
• Limb Deformities, Congenital
• Instabilities, Chromosomal
• Neoplasm Metastasis

• Metastatic Malignant Neoplasm To The Lymph Node
• Oncogene Deregulation
• Peripheral Nerve Injuries
• Tumor Progression
• Neurodegenerative Disorders
• Malignant Neoplasm Of Urinary Bladder
• Manic

Interacting Proteins

• BECN1
• NDC80
• ZW10

Pathways

• Localization
• Chromosome Segregation
• Mitosis
• Kinetochore Localization
• Spindle Checkpoint
• Exocytosis
• Transport
• Anaphase
• Prophase
• Interphase
• Metaphase
• Kinetochore Assembly
• Cell Division

• Cell Cycle
• Spindle Assembly Checkpoint
• Spindle Assembly
• Pathogenesis
• Secretion
• M Phase
• Cytokinesis

PTMs

• Phosphorylation

• Cleavage

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/ZWINT

Boster Bio - Best Uses Of The ZWINT Marker In Lung Cancer

You have likely heard about ZWINT and its importance in the mitotic checkpoint. But did you know that it also plays a role in lung cancer? It is possible to use high-affinity primary antibodies in the treatment of lung cancer. If you don't know about ZWINT, and the potential applications it has in research, click here to learn more. Here are some of its best uses.

ZWINT is an essential component of the mitotic-checkpoint.

It is not clear what role ZWINT plays in lung cancer. Researchers used immunohistochemistry to identify the protein expression in forty samples of lung cancer patients. They also performed Chi-square tests to evaluate correlations between ZWINT mRNA expression and clinicopathological factors, and used a Kaplan-Meier plotter to determine prognostic significance of ZWINT mRNA in lung cancer. Finally, they used Cox proportional-hazards regression analysis to determine if ZWINT expression is an independent risk factor for lung cancer patients, and they analyzed data using STRING database, a database for protein-protein interactions.

ZWINT is essential to kinetochores but also contributes to mitotic-checkpoint signaling. This protein may act as an activation mechanism for MAD2. It is thought to be involved in kinetochore location and interacts with DIC1 as well as p50/dynamitin. It is unknown if ZWINT is involved in chromosome conversion.

In human hepatoma cells, downregulation of the spindle checkpoint protein BUB 1 precedes induction of senescence. BUB 1 is known to modulate the mitotic checkpoint by inhibiting cell death. In addition, apoptosis is accompanied by a proinflammatory response. This research also suggests that ZWINT could regulate DNA damaging agents' sensitivity.

The gene was amplified with IRAUp969B0653D, and LIFESEQ2639836. CPT treatment resulted in both these genes being amplified. These primers also allowed for the amplification of the human Spindly genome. This research indicates that ZWINT may be an important component in the mitotic checkpoint.

It is a possible role in lung cancer

This gene encodes a protein that is involved in the metabolism toxic compounds. It is highly expressed in lung and plays a key role in forming proximal orifices. This gene may be a marker for lung cancer, as it is found in over 80% lung squamous-cell carcinoma patients. The prognosis for lung cancer cells that express this gene is good.

This protein is essential for cell maintenance and cell division. It has also been associated with higher metastatic potential. It has been detected in about 50% of NSCLC patients. Patients with high levels of MCM6 have poor prognosis for lung cancer. It is highly expressed in smokers who smoke cigarettes. Although it is not known if this gene is involved in the pathogenesis or progression of lung cancer, it is a promising marker that could be used for further research.

It is abundant in tumor tissues

The ZWINT Marker is highly expressed in various types of tumor tissues, and is thought to be involved in the biology of tumorigenesis. It was also found to significantly increase in both invasive, and noninvasive forms cancers. However, it was not increased in pediatric T cell acute lymphoblasticleukemia. Its exact role in tumorigenesis will require further research. Although there are conflicting information on this protein, the present study is encouraging and suggests that this gene could be involved in tumorigenesis.

To test the relationship between ZWINT gene expression and lung cancer parameters, two lung subtypes were selected (GSE30219) and GSE31210. In both groups, high ZWINT levels were associated with a higher stage, lower overall survival and an earlier recurrence rate. These results support the idea that ZWINT expression may be high in tumor tissues. This could help in personalized treatment or prevention.

The study showed that ZWINT could be used early as a diagnostic marker for HCC. The protein has also been shown to have a prognostic value, and the study was supported financially by the National Institutes of Health. ZWINT could play a significant part in the diagnosis and treatment for hepatocellular carcinoma, and other forms of liver disease.

ImSig can be applied directly to tumor data. It identifies genetic signatures that help predict the prognosis. The average expression of ImSig signature gene genes is calculated for each type. The median expression value was used to divide patients into two groups. Patients with low levels of B-cells or patients with advanced cancer were excluded. We performed a Cox proportional hazard ratio to determine survival using the R package "survcomp".

It is also available as a primary antibody of high affinity

High-affinity prima antibodies are an excellent way to analyze protein and peptide expression in living cells. Boster offers high-affinity antibodies that have been validated on Western Blotting, Immunohistochemistry, and ELISA. Because of its versatility, scientists can use it in almost any experiment. This antibody can be purchased in 100ul liquid form to allow researchers to quickly purify and measure the protein/peptide they are interested in.

Secondary antibodies are extremely effective at recognizing protein targets. In immunoassays a primary antibody binds specifically to the target protein. This allows researchers more information by detecting a specific level of protein expression. A primary antibody also binds to the same protein with high affinity. These features make secondary antibody valuable tools for determining levels of protein expression in different tissues and cell types.