Product Info Summary
SKU: | A04266-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-ZW10 Antibody Picoband®
SKU/Catalog Number
A04266-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ZW10 Antibody Picoband® catalog # A04266-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-ZW10 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04266-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Standard Protein
E.coli-derived human ZW10 recombinant protein (Position: K35-K779).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A04266-1 is reactive to ZW10 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
95 kDa
Background of ZW10
Centromere/kinetochore protein zw10 homolog is a protein that in humans is encoded by the ZW10 gene This gene encodes a protein that is one of many involved in mechanisms to ensure proper chromosome segregation during cell division. This protein is an essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04266-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Human
Direct ELISA, 0.1-0.5 μg/ml, Human
Positive Control
WB: human Hela whole cell, human K562 whole cell, human Jurkat whole cell, human CACO-2 whole cell, human HL-60 whole cell
FCM: 293T cell
Validation Images & Assay Conditions
![a04266 1 zw10 primary antibodies wb testing 1 a04266 1 zw10 primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a04266-1-zw10-primary-antibodies-wb-testing-1.jpg)
Click image to see more details
Figure 1. Western blot analysis of ZW10 using anti-ZW10 antibody (A04266-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human CACO-2 whole cell lysates,
Lane 5: human HL-60 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZW10 antigen affinity purified polyclonal antibody (Catalog # A04266-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZW10 at approximately 95 kDa. The expected band size for ZW10 is at 89 kDa.
![a04266 1 zw10 primary antibodies fcm testing 2 a04266 1 zw10 primary antibodies fcm testing 2](https://www.bosterbio.com/media/catalog/product/a/0/a04266-1-zw10-primary-antibodies-fcm-testing-2.jpg)
Click image to see more details
Figure 2. Flow Cytometry analysis of 293T cells using anti-ZW10 antibody (A04266-1).
Overlay histogram showing 293T cells stained with A04266-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ZW10 Antibody (A04266-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For ZW10 (Source: Uniprot.org, NCBI)
Gene Name
ZW10
Full Name
Centromere/kinetochore protein zw10 homolog
Weight
Superfamily
ZW10 family
Alternative Names
E3 ubiquitin-protein ligase ZSWIM2; EC 6.3.2.-; MEKK1-related protein X; MEX; MGC33890; Zinc finger SWIM domain-containing protein 2; zinc finger, SWIM domain containing 2; zinc finger, SWIM-type containing 2; ZZZ2ZZ-type zinc finger-containing protein 2 ZW10 HZW10, KNTC1AP zw10 kinetochore protein centromere/kinetochore protein zw10 homolog|ZW10 homolog, centromere/kinetochore protein|ZW10, kinetochore associated, homolog|zeste white 10 homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ZW10, check out the ZW10 Infographic
![ZW10 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ZW10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ZW10 Antibody Picoband® (A04266-1)
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