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Product Info Summary
| SKU: | A05228-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-ZNFN1A2/HELIOS/IKZF2 Antibody Picoband®
SKU/Catalog Number
A05228-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ZNFN1A2/HELIOS/IKZF2 Antibody Picoband® catalog # A05228-3. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ZNFN1A2/HELIOS/IKZF2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05228-3)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human ZNFN1A2/HELIOS/IKZF2 recombinant protein (Position: M1-R477).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A05228-3 is reactive to IKZF2 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
70 kDa
Calculated molecular weight
39411 MW
Background of Helios
Zinc finger protein Helios is a protein that in humans is encoded by the IKZF2 gene. This gene is mapped to 2q34.This gene encodes a member of the Ikaros family of zinc-finger proteins. Three members of this protein family (Ikaros, Aiolos and Helios) are hematopoietic-specific transcription factors involved in the regulation of lymphocyte development. This protein forms homo- or hetero-dimers with other Ikaros family members, and is thought to function predominantly in early hematopoietic development. Multiple transcript variants encoding different isoforms have been found for this gene, but the biological validity of some variants has not been determined.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05228-3 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human Hela whole cell, human K562 whole cell, human Jurkat whole cell, human Raji whole cell, rat spleen tissue, mouse ANA-1 whole cell
FCM: HL-60 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of IKZF2 using anti-IKZF2 antibody (A05228-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human Raji whole cell lysates,
Lane 5: rat spleen tissue lysates,
Lane 6: mouse ANA-1 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IKZF2 antigen affinity purified polyclonal antibody (Catalog # A05228-3) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IKZF2 at approximately 70 kDa. The expected band size for IKZF2 is at 60 kDa.
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Figure 2. Flow Cytometry analysis of HL-60 cells using anti-IKZF2 antibody (A05228-3).
Overlay histogram showing HL-60 cells stained with A05228-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IKZF2 Antibody (A05228-3, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For IKZF2 (Source: Uniprot.org, NCBI)
Gene Name
IKZF2
Full Name
Zinc finger protein Helios
Weight
39411 MW
Superfamily
Ikaros C2H2-type zinc-finger protein family
Alternative Names
Zinc finger protein Helios; Ikaros family zinc finger protein 2; IKZF2; HELIOS, ZNFN1A2 Ikzf2|A730095J18Rik, He, Helios, Zfpn, Zfpn1a2, Znfn1a2|IKAROS family zinc finger 2|zinc finger protein Helios|ikaros family zinc finger protein 2|zinc finger protein, subfamily 1A, 2 (Helios)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IKZF2, check out the IKZF2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IKZF2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ZNFN1A2/HELIOS/IKZF2 Antibody Picoband® (A05228-3)
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