Product Info Summary
SKU: | A11267-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-ZNF318 Antibody Picoband™
SKU/Catalog Number
A11267-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ZNF318 Antibody Picoband™ catalog # A11267-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-ZNF318 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11267-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human ZNF318 recombinant protein (Position: E680-Q1197).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A11267-1 is reactive to ZNF318 in Human, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
290 kDa
Calculated molecular weight
Background of ZNF318
Zinc finger protein 318 is a protein that in humans is encoded by the ZNF318 gene. ZNF318 encodes a nuclear protein with a zinc finger motif of the Cys2-His2 type that is a novel corepressor of androgen receptor (AR).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11267-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
Direct ELISA, 0.1-0.5 μg/ml, Human
Positive Control
Validation Images & Assay Conditions
![a11267 1 znf318 primary antibodies wb testing 1 a11267 1 znf318 primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/a/1/a11267-1-znf318-primary-antibodies-wb-testing-1.jpg)
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Figure 1. Western blot analysis of ZNF318 using anti-ZNF318 antibody (A11267-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human THP-1 whole cell lysates,
Lane 2: rat stomach tissue lysates,
Lane 3: rat C6 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZNF318 antigen affinity purified polyclonal antibody (Catalog # A11267-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZNF318 at approximately 290 kDa. The expected band size for ZNF318 is at 251 kDa.
![a11267 1 znf318 primary antibodies fcm testing 2 a11267 1 znf318 primary antibodies fcm testing 2](https://www.bosterbio.com/media/catalog/product/a/1/a11267-1-znf318-primary-antibodies-fcm-testing-2.png)
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Figure 2. Flow Cytometry analysis of A431 cells using anti-ZNF318 antibody (A11267-1).
Overlay histogram showing A431 cells stained with A11267-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ZNF318 Antibody (A11267-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For ZNF318 (Source: Uniprot.org, NCBI)
Gene Name
ZNF318
Full Name
Zinc finger protein 318
Weight
Alternative Names
Zinc finger and SCAN domain-containing protein 15; zinc finger protein 397; Zinc finger protein 47ZSCAN15MGC13250; ZNF47 ZNF318 HRIHFB2436, TZF, ZFP318 zinc finger protein 318 zinc finger protein 318|endocrine regulator|endocrine regulatory protein|testicular zinc finger
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ZNF318, check out the ZNF318 Infographic
![ZNF318 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ZNF318: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ZNF318 Antibody Picoband™ (A11267-1)
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