Anti-ZNF147 TRIM25 Antibody

Product Name

Anti-ZNF147 TRIM25 Antibody

View all TRIM25 Antibodies

SKU/Catalog Number

A03232

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-ZNF147 TRIM25 Antibody (Catalog # A03232). Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ZNF147 TRIM25 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03232)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human ZNF147.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A03232 is reactive to TRIM25 in Human

Applications

A03232 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

70973 MW

Background of TRIM25

Functions as a ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs.

Inoue S., Proc. Natl. Acad. Sci. U.S.A. 90:11117-11121(1993).
The MGC Project Team, Genome Res. 14:2121-2127(2004).
Rush J., Nat. Biotechnol. 23:94-101(2005).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Validation Images & Assay Conditions

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Western blot analysis of extracts from K562 cells, using ZNF147 antibody A03232.

Gene/Protein Information For TRIM25 (Source: Uniprot.org, NCBI)

Alternative Names

EC 6.3.2.19; EC 6.3.2.n3; EFPZ147; Estrogen-responsive finger protein; RING finger protein 147; RNF147E3 ubiquitin/ISG15 ligase TRIM25; tripartite motif containing 25; tripartite motif-containing 25; Tripartite motif-containing protein 25; Ubiquitin/ISG15-conjugating enzyme TRIM25; zinc finger protein 147 (estrogen-responsive finger protein); Zinc finger protein 147; zinc finger protein-147; ZNF147tripartite motif protein TRIM25 TRIM25 EFP, RNF147, Z147, ZNF147 tripartite motif containing 25 E3 ubiquitin/ISG15 ligase TRIM25|RING finger protein 147|RING-type E3 ubiquitin transferase TRIM25|estrogen-responsive finger protein|tripartite motif protein TRIM25|tripartite motif-containing protein 25|ubiquitin/ISG15-conjugating enzyme TRIM25|zinc finger protein 147 (estrogen-responsive finger protein)|zinc finger protein-147

For more info on TRIM25, check out the TRIM25 Infographic

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In this infographic, you will see the following information for TRIM25: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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