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Product Info Summary
| SKU: | A03232-2 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-TRIM25 Antibody
SKU/Catalog Number
A03232-2
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-TRIM25 Antibody (Catalog # A03232-2). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
TRIM25 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-TRIM25 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03232-2)
Host
Rabbit
Contents
TRIM25 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
TRIM25 antibody was raised against a 19 amino acid synthetic peptide from near the carboxy terminus of human TRIM25. The immunogen is located within amino acids 520 - 570 of TRIM25.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reaction to other death receptors.
Reactive Species
A03232-2 is reactive to TRIM25 in Human, Mouse
Applications
A03232-2 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
70973 MW
Background of TRIM25
TRIM25, also known as estrogen responsive finger protein (EFP) is a member of the RING finger-B box-coiled coil family and is a downstream target of estrogen receptor a. Mice lacking this gene displayed an underdeveloped uterus and reduced estrogen responsiveness demonstrating that this protein is essential for estrogen dependent proliferation. TRIM25 expression is increased in breast carcinoma where it promotes the growth of breast tumor by functioning as an E3 ubiquitin ligase that targets the negative cell cycle checkpoint 14-3-3s. Recent reports have indicated that TRIM25 E3 ubiquitin ligase is essential for the retinoic-acid-inducible gene 1 (RIG-1) signaling pathway to elicit host antiviral innate immunity.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
TRIM25 antibody can be used for detection of TRIM25 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of TRIM25 in HeLa cell lysate in (A) the absence and (B) presence of blocking peptide with TRIM25 antibody at 1 μg/mL.
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Immunocytochemistry of TRIM25 in HeLa cells with TRIM25 antibody at 20 μg/mL.
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Immunofluorescence of TRIM25 in HeLa cells with TRIM25 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For TRIM25 (Source: Uniprot.org, NCBI)
Gene Name
TRIM25
Full Name
E3 ubiquitin/ISG15 ligase TRIM25
Weight
70973 MW
Alternative Names
EC 6.3.2.19; EC 6.3.2.n3; EFPZ147; Estrogen-responsive finger protein; RING finger protein 147; RNF147E3 ubiquitin/ISG15 ligase TRIM25; tripartite motif containing 25; tripartite motif-containing 25; Tripartite motif-containing protein 25; Ubiquitin/ISG15-conjugating enzyme TRIM25; zinc finger protein 147 (estrogen-responsive finger protein); Zinc finger protein 147; zinc finger protein-147; ZNF147tripartite motif protein TRIM25 TRIM25 EFP, RNF147, Z147, ZNF147 tripartite motif containing 25 E3 ubiquitin/ISG15 ligase TRIM25|RING finger protein 147|RING-type E3 ubiquitin transferase TRIM25|estrogen-responsive finger protein|tripartite motif protein TRIM25|tripartite motif-containing protein 25|ubiquitin/ISG15-conjugating enzyme TRIM25|zinc finger protein 147 (estrogen-responsive finger protein)|zinc finger protein-147
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TRIM25, check out the TRIM25 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TRIM25: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TRIM25 Antibody (A03232-2)
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