Product Info Summary
SKU: | A07719-2 |
---|---|
Size: | 0.1 mg |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-ZIP7 SLC39A7 Antibody
View all SLC39A7/ZIP7 Antibodies
SKU/Catalog Number
A07719-2
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-ZIP7 SLC39A7 Antibody (Catalog # A07719-2). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
ZIP7 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-ZIP7 SLC39A7 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07719-2)
Host
Rabbit
Contents
ZIP7 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-ZIP7 antibody was raised against a peptide corresponding to 17 amino acids near the amino terminus of human ZIP7. The immunogen is located within amino acids 20-70 of ZIP7.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
ULK3 antibody is human specific. Multiple isoforms of ULK3 are known to exist.
Reactive Species
A07719-2 is reactive to SLC39A7 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
50118 MW
Background of SLC39A7/ZIP7
The zinc transporter ZIP7, also known as SLC39A7, is a member of a family of divalent ion transporters. Zinc is an essential ion for cells and plays significant roles in the growth, development, and differentiation. ZIP7 was initially identified while characterizing genes in the major histocompatibility complex on chromosome 17. ZIP7 mRNA is abundantly and widely expressed and the protein localizes to the Golgi apparatus. It functions to transport intracellular zinc from the Golgi apparatus to the cytoplasm of the cell. ZIP7 expression is expressed by zinc. ZIP7 has been suggested to act a hub for tyrosine kinase activation and may thus be a potential therapeutic target for diseases such as cancer where prevention of tyrosine kinase activation would be advantageous.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07719-2 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 0.5-1 μg/mL; IHC: 2.5 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
Click image to see more details
Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines
Loading: 15 μg of lysates per lane.Antibodies: ZIP7, A07719-2 (1 μg/mL), ZIP7, (4 μg/mL), and beta-actin (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Click image to see more details
Western Blot Validation in Human Cell Lines
Loading: 15 μg of lysates per lane.Antibodies: ZIP7, A07719-2 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Click image to see more details
Western Blot Validation in Human Brain Tissue and 3T3 (Balb) Cell Lysate
Loading: 15 μg of lysates per lane. Antibodies: ZIP7, A07719-2 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Click image to see more details
Western Blot Validation in Mouse Brain Tissue Lysate
Loading: 15 μg of lysates per lane. Antibodies: ZIP7
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Western Blot Validation in Rat Brain Tissue Lysate
Loading: 15 μg of lysates per lane.Antibodies: ZIP7
Click image to see more details
Immunohistochemistry Validation of ZIP7 in Human Brain Tissue
Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-ZIP7 antibody (A07719-2) at 2.5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Click image to see more details
Immunofluorescence Validation of ZIP7 in Human Brain Tissue
Immunofluorescent analysis of 4% paraformaldehyde-fixed human brain tissue labeling ZIP7 with A07719-2 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
Protein Target Info & Infographic
Gene/Protein Information For SLC39A7 (Source: Uniprot.org, NCBI)
Gene Name
SLC39A7
Full Name
Zinc transporter SLC39A7
Weight
50118 MW
Superfamily
ZIP transporter (TC 2.A.5) family
Alternative Names
KE4, HKE4, ZIP7, RING5, H2-KE4, D6S115E, D6S2244E, Zinc transporter SLC39A7, Histidine-rich membrane protein Ke4 SLC39A7 D6S115E, D6S2244E, H2-KE4, HKE4, KE4, RING5, ZIP7 solute carrier family 39 member 7 zinc transporter SLC39A7|HLA class II region expressed gene KE4|Ke4 gene, mouse, human homolog of|histidine-rich membrane protein Ke4|really interesting new gene 5 protein|solute carrier family 39 (zinc transporter), member 7|zrt-, Irt-like protein 7
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SLC39A7, check out the SLC39A7 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SLC39A7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ZIP7 SLC39A7 Antibody (A07719-2)
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