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Product Info Summary
| SKU: | A03300-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-ZIP Kinase/Dapk3 Antibody Picoband®
View all DAPK3/ZIPK Antibodies
SKU/Catalog Number
A03300-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ZIP Kinase/Dapk3 Antibody Picoband® catalog # A03300-3. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-ZIP Kinase/Dapk3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03300-3)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse ZIP Kinase/Dapk3 recombinant protein (Position: R290-R448).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03300-3 is reactive to Dapk3 in Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
53 kDa
Calculated molecular weight
52.536kDa
Background of DAPK3/ZIPK
Death-associated protein kinase 3 is an enzyme that in humans is encoded by the DAPK3 gene. Death-associated protein kinase 3 (DAPK3) induces morphological changes in apoptosis when overexpressed in mammalian cells. These results suggest that DAPK3 may play a role in the induction of apoptosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03300-3 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Mouse
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: mouse lung tissue, mouse stomach tissue, rat kidney tissue, rat PC-12 whole cell
ICC/IF: RM-1 cell
FCM: HEPA1-6 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of ZIP Kinase/Dapk3 using anti-ZIP Kinase/Dapk3 antibody (A03300-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse lung tissue lysates,
Lane 2: mouse stomach tissue lysates,
Lane 3: rat kidney tissue lysates,
Lane 4: rat PC-12 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZIP Kinase/Dapk3 antigen affinity purified polyclonal antibody (Catalog # A03300-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZIP Kinase/Dapk3 at approximately 53 kDa. The expected band size for ZIP Kinase/Dapk3 is at 53 kDa.
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Figure 2. Flow Cytometry analysis of HEPA1-6 cells using anti-ZIP Kinase/Dapk3 antibody (A03300-3).
Overlay histogram showing HEPA1-6 cells stained with A03300-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ZIP Kinase/Dapk3 Antibody (A03300-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Figure 3. IF analysis of ZIP Kinase/Dapk3 using anti-ZIP Kinase/Dapk3 antibody (A03300-3).
ZIP Kinase/Dapk3 was detected in an immunocytochemical section of RM-1 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ZIP Kinase/Dapk3 Antibody (A03300-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For Dapk3 (Source: Uniprot.org, NCBI)
Gene Name
Dapk3
Full Name
Death-associated protein kinase 3
Weight
52.536kDa
Superfamily
protein kinase superfamily
Alternative Names
Glutathione S-transferase Mu 3; GST class-mu 3; GSTM3-3; hGSTM3-3; GSTM3; GST5; DAPK3 DLK, ZIP, ZIPK death associated protein kinase 3 death-associated protein kinase 3|DAP kinase 3|DAP-like kinase|MYPT1 kinase|ZIP-kinase|zipper-interacting protein kinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Dapk3, check out the Dapk3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Dapk3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ZIP Kinase/Dapk3 Antibody Picoband® (A03300-3)
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