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Product Info Summary
| SKU: | A13494 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IHC |
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Product info
Product Name
Anti-ZC3H4 Antibody
SKU/Catalog Number
A13494
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-ZC3H4 Antibody (Catalog # A13494). Tested in IHC applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ZC3H4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13494)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human ZC3H4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A13494 is reactive to ZC3H4 in Human, Mouse
Reconstitution
Calculated molecular weight
140257 MW
Background of ZC3H4
This gene encodes a member of a family of CCCH (C-x8-C-x5-C-x3-H type) zinc finger domain-containing proteins. These zinc finger domains, which coordinate zinc finger binding and are characterized by three cysteine residues and one histidine residue, are nucleic acid-binding. Other family members are known to function in post-transcriptional regulation.
Kikuno R., DNA Res. 6:197-205(1999).
Nakajima D., DNA Res. 9:99-106(2002).
The German cDNA consortium; Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
Beausoleil S.A., Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A13494 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using ZC3H4 antibody A13494.
Protein Target Info & Infographic
Gene/Protein Information For ZC3H4 (Source: Uniprot.org, NCBI)
Gene Name
ZC3H4
Full Name
Zinc finger CCCH domain-containing protein 4
Weight
140257 MW
Alternative Names
Zinc finger CCCH domain-containing protein 4;ZC3H4;C19orf7, KIAA1064; ZC3H4 C19orf7 zinc finger CCCH-type containing 4 zinc finger CCCH domain-containing protein 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ZC3H4, check out the ZC3H4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ZC3H4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ZC3H4 Antibody (A13494)
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No publications found for A13494
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Customer Q&As
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7 Customer Q&As for Anti-ZC3H4 Antibody
Question
We are currently using anti-ZC3H4 antibody A13494 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-04-15
Answer
The anti-ZC3H4 antibody (A13494) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-04-15
Question
My question regarding product A13494, anti-ZC3H4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-03-07
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A13494 anti-ZC3H4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-03-07
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for forebrain using anti-ZC3H4 antibody A13494. Let me know if you need anything else.
C. Krishna
Verified customer
Asked: 2018-11-20
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-11-20
Question
I was wanting to use your anti-ZC3H4 antibody for IHC for mouse forebrain on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse forebrain identification?
L. Anderson
Verified customer
Asked: 2018-10-11
Answer
As indicated on the product datasheet, A13494 anti-ZC3H4 antibody has been tested for IHC on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse forebrain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-10-11
Question
Please see the WB image, lot number and protocol we used for forebrain using anti-ZC3H4 antibody A13494. Please let me know if you require anything else.
D. Gonzalez
Verified customer
Asked: 2017-08-31
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-08-31
Question
Will anti-ZC3H4 antibody A13494 work on primate IHC with forebrain?
A. Yang
Verified customer
Asked: 2015-11-25
Answer
Our lab technicians have not validated anti-ZC3H4 antibody A13494 on primate. You can run a BLAST between primate and the immunogen sequence of anti-ZC3H4 antibody A13494 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate forebrain in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-11-25
Question
Is this A13494 anti-ZC3H4 antibody reactive to the isotypes of ZC3H4?
E. Li
Verified customer
Asked: 2015-10-06
Answer
The immunogen of A13494 anti-ZC3H4 antibody is Synthesized peptide derived from internal of human ZC3H4. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-10-06
