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Product Info Summary
| SKU: | A12536 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-ZBTB8 Antibody
SKU/Catalog Number
A12536
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-ZBTB8 Antibody (Catalog # A12536). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
ZBTB8 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-ZBTB8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12536)
Host
Rabbit
Contents
ZBTB8 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
ZBTB8 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human ZBTB8. The immunogen is located within the last 50 amino acids of ZBTB8.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least two isoforms of ZBTB8 are known to exist; this antibody will recognize both isoforms. This antibody is predicted to not cross-react with other ZBTB protein family members.
Reactive Species
A12536 is reactive to ZBTB8A in Human, Mouse
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
50141 MW
Background of Zbtb8a
The ZBTB family of proteins is comprised of diverse zinc finger proteins that also contain a BTB (BR-C, ttk and bab) domain. ZBTB8, also known as BOZ-F1, is present in multiple tissues, and is highly expressed in the cytoplasm of the placenta. While little is known about ZBTB8, other ZBTB proteins, such as ZBTB4 bind methylated DNA and repress transcription. Another ZBTB proteins, ZBTB7A, has been implicated as a proto-oncogene whose overexpression contributes to malignancy in breast cancer, suggesting that ZBTB8 may act as a transcriptional repressor or be involved in tumorigenesis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A12536 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ZBTB8 antibody can be used for detection of ZBTB8 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of ZBTB8 in mouse spleen tissue lysate with ZBTB8 antibody at 1 μg/mL.
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Immunohistochemistry of ZBTB8 in human spleen tissue with ZBTB9 antibody at 2.5 μg/mL.
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Immunofluorescence ofZBTB8 in human spleen tissue with ZBTB8 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For ZBTB8A (Source: Uniprot.org, NCBI)
Gene Name
ZBTB8A
Full Name
Zinc finger and BTB domain-containing protein 8A
Weight
50141 MW
Alternative Names
BOZF1, ZBTB8, ZNF916A, BOZF1, Zinc finger and BTB domain-containing protein 8A, BOZ-F1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ZBTB8A, check out the ZBTB8A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ZBTB8A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ZBTB8 Antibody (A12536)
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No publications found for A12536
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