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Product Info Summary
| SKU: | M03081 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-ZBTB7A Rabbit Monoclonal Antibody
View all ZBTB7A/Pokemon Antibodies
SKU/Catalog Number
M03081
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-ZBTB7A Rabbit Monoclonal Antibody catalog # M03081. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ZBTB7A Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03081)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
23Z22
Isotype
IgG
Immunogen
A synthesized peptide derived from human ZBTB7A
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M03081 is reactive to ZBTB7A in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
78 kDa
Calculated molecular weight
53543 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03081 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:5000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
Positive Control
WB: human HepG2 whole cell, human A431 whole cell, human MCF-7 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of ZBTB7A using anti-ZBTB7A antibody (M03081).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZBTB7A antigen affinity purified monoclonal antibody (Catalog # M03081) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZBTB7A at approximately 75 kDa. The expected band size for ZBTB7A is at 61 kDa.
Protein Target Info & Infographic
Gene/Protein Information For ZBTB7A (Source: Uniprot.org, NCBI)
Gene Name
ZBTB7A
Full Name
Zinc finger and BTB domain-containing protein 7A
Weight
53543 MW
Alternative Names
TNF receptor-associated factor 4;Cysteine-rich domain associated with RING and Traf domains protein 1;Metastatic lymph node gene 62 protein;MLN 62;RING finger protein 83;TRAF4;CART1, MLN62, RNF83; Zbtb7a|9030619K07Rik, 9130006G12Rik, AI452336, FBI-, FBI-1, L, Lrf, Pok, Pokemon, Zbtb7|zinc finger and BTB domain containing 7a|zinc finger and BTB domain-containing protein 7A|POK erythroid myeloid ontogenic factor|POZ and Krueppel erythroid myeloid ontogenic factor|leukemia/lymphoma related factor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ZBTB7A, check out the ZBTB7A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ZBTB7A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ZBTB7A Rabbit Monoclonal Antibody (M03081)
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No publications found for M03081
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