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Product Info Summary
| SKU: | A14543 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-ZBTB5 Antibody
SKU/Catalog Number
A14543
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-ZBTB5 Antibody (Catalog # A14543). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
ZBTB5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-ZBTB5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14543)
Host
Rabbit
Contents
ZBTB5 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
ZBTB5 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human ZBTB6. The immunogen is located within the last 50 amino acids of ZBTB5.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least two isoforms of ZBTB5 are known to exist; this antibody will recognize both isoforms. This antibody is predicted to not cross-react with other ZBTB protein family members.
Reactive Species
A14543 is reactive to ZBTB5 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
74278 MW
Background of ZBTB5
The ZBTB family of proteins is comprised of diverse zinc finger proteins that also contain a BTB (BR-C, ttk and bab) domain. ZBTB5 was identified though sequence analysis as a POZ domain Kruppel-like zinc finger (POK) protein. Further experiments indicated that it binds DNA and can directly repress transcription of the cell cycle arrest gene p21. ZBTB5 can also interact with co-repressor histone deacetylase complexes such as BCoR, NCoR, and SMRT via its POZ domain, resulting in deacetylation of histones Ac-H3 and Ac-H4 at the proximal promoter. ZBTB5 stimulates both cell proliferation and cell cycle progression, suggesting that it may act as a potential proto-oncogene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A14543 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ZTBT5 antibody can be used for detection of ZTBT5 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of ZBTB5 in mouse brain tissue lysate with ZBTB5 antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of ZBTB5 in human brain tissue with ZBTB5 antibody at 2.5 μg/mL.
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Immunofluorescence of ZBTB5 in human brain tissue with ZBTB5 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For ZBTB5 (Source: Uniprot.org, NCBI)
Gene Name
ZBTB5
Full Name
Zinc finger and BTB domain-containing protein 5
Weight
74278 MW
Alternative Names
KIAA0354, Zinc finger and BTB domain-containing protein 5 ZBTB5 zinc finger and BTB domain containing 5 zinc finger and BTB domain-containing protein 5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ZBTB5, check out the ZBTB5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ZBTB5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ZBTB5 Antibody (A14543)
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No publications found for A14543
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