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Product Info Summary
| SKU: | A08155 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-ZBTB4 Antibody
SKU/Catalog Number
A08155
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-ZBTB4 Antibody (Catalog # A08155). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
ZBTB4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-ZBTB4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08155)
Host
Rabbit
Contents
ZBTB4 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
ZBTB4 antibody was raised against a 18 amino acid synthetic peptide near the carboxy terminus of human ZBTB4. The immunogen is located within amino acids 790 - 840 of ZBTB4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least four isoforms of ZBTB4 are known to exist; this antibody will only recognize the longest isoform. This antibody is predicted to not cross-react with other ZBTB protein family members.
Reactive Species
A08155 is reactive to ZBTB4 in Human, Mouse
Applications
A08155 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
105114 MW
Background of ZBTB4
The ZBTB family of proteins is comprised of diverse zinc finger proteins that also contain a BTB (BR-C, ttk and bab) domain. Similar to Kaiso, a zinc-finger containing protein that can bind methylated CpGs, ZBTB4 can also bind methylated DNA and repress transcription. ZBTB4 has been shown to associate with the Sin3/histone deacetylase co-repressor and repress expression of P21CIP1 as part of a heterodimeric complex with Miz1. In cultured cells, depletion of ZBTB4 promotes cell cycle arrest in response to p53 activation and suppresses apoptosis through regulation of P21CIP1, suggesting that ZBTB4 is a critical determinant of the cellular response to p53 activation. HIPK2, a kinase that is involved in cellular proliferation and survival, phosphorylates and down-regulates ZBTB4 under normal cell growth conditions; this degradation increases with DNA damage.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ZBTB4 antibody can be used for detection of ZBTB4 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of ZBTB4 in SK-N-SH cell lysate with ZBTB4 antibody at 1 μg/mL.
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Immunohistochemistry of ZBTB4 in human brain tissue with ZBTB4 antibody at 2.5 μg/mL.
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Immunofluorescence of ZBTB4 in human brain tissue with ZBTB4 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For ZBTB4 (Source: Uniprot.org, NCBI)
Gene Name
ZBTB4
Full Name
Zinc finger and BTB domain-containing protein 4
Weight
105114 MW
Alternative Names
KAISO-L1; zinc finger and BTB domain containing 4 ZBTB4 KAISO-L1, ZNF903 zinc finger and BTB domain containing 4 zinc finger and BTB domain-containing protein 4|KAISO-like zinc finger protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ZBTB4, check out the ZBTB4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ZBTB4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ZBTB4 Antibody (A08155)
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