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Product Info Summary
| SKU: | PB9909 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-YY1 Antibody Picoband®
SKU/Catalog Number
PB9909
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-YY1 Antibody Picoband® catalog # PB9909. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-YY1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9909)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human YY1, identical to the related mouse sequence.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9909 is reactive to YY1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
65 kDa
Calculated molecular weight
44713 MW
Background of YY1
YY1 (Yin Yang 1) is a transcriptional repressor protein in humans that is encoded by the YY1 gene. YY1 is a ubiquitously distributed transcription factor belonging to the GLI-Kruppel class of zinc finger proteins. The protein is involved in repressing and activating a diverse number of promoters. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the function of YY1.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9909 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Positive Control
WB: mouse thymus tissue, mouse spleen tissue, rat thymus tissue, rat PC-12 whole cell, mouse RAW2647 whole cell, human PC-3 whole cell, human SW620 whole cell
ICC/IF: A431 cell
FCM: THP-1 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of YY1 using anti-YY1 antibody (PB9909).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse thymus tissue lysates,
Lane 2: mouse spleen tissue lysates,
Lane 3: rat thymus tissue lysates,
Lane 4: rat PC-12 whole cell lysates,
Lane 5: mouse RAW264.7 whole cell lysates,
Lane 6: human PC-3 whole cell lysates,
Lane 7: human SW620 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-YY1 antigen affinity purified polyclonal antibody (Catalog # PB9909) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for YY1 at approximately 65 kDa. The expected band size for YY1 is at 45 kDa.
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Figure 2. IF analysis of YY1 using anti-YY1 antibody (PB9909).
YY1 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-YY1 Antibody (PB9909) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 3. Flow Cytometry analysis of THP-1 cells using anti-YY1 antibody (PB9909).
Overlay histogram showing THP-1 cells stained with PB9909 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-YY1 Antibody (PB9909,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For YY1 (Source: Uniprot.org, NCBI)
Gene Name
YY1
Full Name
Transcriptional repressor protein YY1
Weight
44713 MW
Superfamily
YY transcription factor family
Alternative Names
Transcriptional repressor protein YY1;Delta transcription factor;INO80 complex subunit S;NF-E1;Yin and yang 1;YY-1;YY1;INO80S; YY1 DELTA, GADEVS, INO80S, NF-E1, UCRBP, YIN-YANG-1 YY1 transcription factor transcriptional repressor protein YY1|INO80 complex subunit S|YY-1|Yin and Yang 1 protein|delta transcription factor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on YY1, check out the YY1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for YY1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-YY1 Antibody Picoband® (PB9909)
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Customer Q&As
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4 Customer Q&As for Anti-YY1 Antibody Picoband®
Question
We are currently using anti-YY1 antibody PB9909 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on bovine tissues as well?
C. Jones
Verified customer
Asked: 2020-02-26
Answer
The anti-YY1 antibody (PB9909) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-26
Question
I see that the anti-YY1 antibody PB9909 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-11-11
Answer
You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-11-11
Question
My question regarding product PB9909, anti-YY1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-07
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9909 anti-YY1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-07
Question
Is a blocking peptide available for product anti-YY1 antibody (PB9909)?
C. Baker
Verified customer
Asked: 2018-05-16
Answer
We do provide the blocking peptide for product anti-YY1 antibody (PB9909). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-05-16
