Click image to see more details
Product Info Summary
| SKU: | A06172-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-YTHDC1 Antibody Picoband®
SKU/Catalog Number
A06172-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-YTHDC1 Antibody Picoband® catalog # A06172-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-YTHDC1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06172-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.005mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human YTHDC1 recombinant protein (Position: S326-K651).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A06172-1 is reactive to YTHDC1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Observed Molecular Weight
85 kDa
Calculated molecular weight
84.7kDa
Background of YTHDC1
YTH domain-containing protein 1 is a protein that in humans is encoded by the YTHDC1 gene. YTHDC1 is a nuclear protein involved in splice site selection that localises to YT bodies; dynamic subnuclear compartments, which first appear at the beginning of S-phase in the cell cycle and disperse during mitosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06172-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human placenta tissue, human U937 whole cell, human K562 whole cell, human HEPG2 whole cell, human HELA whole cell, human Jurkat whole cell, rat testis tissue, rat C6 whole cell, mouse testis tissue, mouse RAW2647 whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of YTHDC1 using anti-YTHDC1 antibody (A06172-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human U937 whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human HEPG2 whole cell lysates,
Lane 5: human HELA whole cell lysates,
Lane 6: human Jurkat whole cell lysates,
Lane 7: rat testis tissue lysates,
Lane 8: rat C6 whole cell lysates,
Lane 9: mouse testis tissue lysates,
Lane 10: mouse RAW264.7 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-YTHDC1 antigen affinity purified polyclonal antibody (Catalog # A06172-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for YTHDC1 at approximately 85KD. The expected band size for YTHDC1 is at 85KD.
Protein Target Info & Infographic
Gene/Protein Information For YTHDC1 (Source: Uniprot.org, NCBI)
Gene Name
YTHDC1
Full Name
YTH domain-containing protein 1
Weight
84.7kDa
Alternative Names
CD160 antigen; Natural killer cell receptor BY55; CD160; BY55 YTHDC1 YT521, YT521-B YTH domain containing 1 YTH domain-containing protein 1|putative splicing factor YT521|splicing factor YT521|splicing factor YT521-B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on YTHDC1, check out the YTHDC1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for YTHDC1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-YTHDC1 Antibody Picoband® (A06172-1)
Hello CJ!
No publications found for A06172-1
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-YTHDC1 Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-YTHDC1 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
