Y-box-binding protein 1 Antibodies

Y-box-binding protein 1 (YBX1)

Mediates pre-mRNA alternative splicing regulation. Binds to splice sites in pre-mRNA and modulates splice site selection.

Contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors (By similarity). Regulates the transcription of numerous genes.

Gene Information

Human
Gene Name:	YBX1
Uniprot:	P67809
Entrez:	4904

Family

Belongs to:
YBX1 family

Alternative Names

BP-8; CBF-A; class II, Y box-binding protein I; CSDA2; CSDB; DBPB CCAAT-binding transcription factor I subunit A; EFI-A; Enhancer factor I subunit A; MDR-NF1; MGC104858; MGC110976; MGC117250; NSEP1 Y-box-binding protein 1; nuclease-sensitive element-binding protein 1; Y box binding protein 1; YB1 DNA-binding protein B; YB-1 Y-box transcription factor; YBX1

Molecular Weight

Mass (kDA):
35.924 kDA

Genomic Context

Human
Location:	1p34.2
Sequence:	1; NC_000001.11 (42682418..42703805)

Subcellular Localizations

Cytoplasm. Nucleus. Cytoplasmic granule. Secreted. Secreted, extracellular exosome. Predominantly cytoplasmic in proliferating cells (PubMed:12604611). Cytotoxic stress and DNA damage enhance translocation to the nucleus (PubMed:14718551). Localized in cytoplasmic mRNP granules containing untranslated mRNAs (PubMed:25229427). Shuttles between nucleus and cytoplasm (PubMed:25229427). Localized with DDX1, MBNL1 and TIAL1 in stress granules upon stress (PubMed:18335541). Secreted by mesangial and monocytic cells after inflammatory challenges (PubMed:19483673).

Diseases

• Neoplasms
• Malignant Neoplasms
• Mammary Neoplasms
• Neurofibromatosis 1
• Malignant Neoplasm Of Breast
• Carcinoma
• Malignant Paraganglionic Neoplasm
• Cell Transformation, Neoplastic
• Liver Carcinoma
• Neoplasm Metastasis
• Cell Invasion
• Leukemia
• Genotoxic Stress

• Glioma
• Colonic Neoplasms
• Sarcoma
• Adenocarcinoma
• Leukoencephalopathies
• Malignant Neoplasm Of Lung
• Nervousness

Interacting Proteins

• C1QBP
• DHX9
• EP300
• FBXO33
• LSM3

• PITX2
• PPM1G
• TPT1

Pathways

• Dna Replication
• Translation
• Localization
• Drug Resistance
• Cell Proliferation
• Methylation
• Dna Repair
• Cell Cycle
• Cell Growth
• Pathogenesis
• Regulation Of Gene Expression
• Transport
• Rna Interference

• Viral Replication
• Chromatin Remodeling
• Gene Silencing
• Rna Splicing
• Single-stranded Dna Binding
• Rna Processing
• Lactation

PTMs

• Phosphorylation
• Methylation
• Cleavage
• Acetylation
• Reduction

• Glycosylation
• Oxidation
• Demethylation
• Farnesylation
• Dephosphorylation

• Ubiquitination

Research Areas

• Breast Cancer
• Cancer
• DNA Repair
• Epigenetics
• Ovarian Carcinoma Cell Markers

• Stem Cell Markers
• Transcription Factors and Regulators
• Translation Control

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/YBX1

Boster Bio - Best Uses Of The YBX1 Marker

The YBX1 mark is part of the cold shock protein family. It has been confirmed in a variety of studies, including breast cancer. In this article, we'll examine the most effective applications of this marker. We will also talk about the validity of this marker in a variety of samples and the many benefits. This article will review the YBX1 marker and its advantages in the field of breast cancer.

YBX1 belongs to the cold shock protein family.

YBX1 belongs to the cold shock protein family. It is a family of proteins that regulate the expression of genes in cells in malignant and inflammation. It has been discovered to modulate Jak2 signaling in a variety of cancers, including leukemia, and is also known to shield cells with Jak2-mutation from the harmful effects of jak inhibitors.

The YBX1 gene is an important RNA-binding protein involved in recruiting MYC transcripts into polysome chains. Knockout mice lacking YBX1 experience a loss of gene dependence in their mRNA fractions. Two sgRNAs which target the YBX1 genes, reduce MYC expression in MOLM-13 cells. The RNA-tape bands show that YBX1 binding depends on the dose of the gene.

The transcription factor YB-1 that targets most cellular compartments. It is involved in regulating a variety of key cellular functions, including the regulation and translation of mRNA. YB-1 is necessary for pre-mRNA splicingand storage of mRNA, and mRNA transcriptional mRNPs that are inactive have significantly less YB-1 content than polysomal mRNPs. It also regulates the translation process by enhancing Twist Snail and HIF1a. It may be relevant for epithelial-mesenchymal transition, a transition from epithelial to mesenchymal tissue.

Knockout mice infected by YBX1 The RNA has a distinct the morphology of their bodies and death. YBX1 is RNA-seq demonstrated a different enhancement of the gene-targeting protein in cells expressing the altered YBX1 protein. YBX1 is associated with genes that are involved in innate immunity, cell cycle arrest and splicing CD44 alternative exon v4.

It is a unique biomarker for breast cancer

The high level of expression of YBX1 in breast cancer cells was linked with worse outcomes than those with low expression. YBX1 and its associated gene CTPS1 may be useful as biomarkers in breast cancer. Researchers examined the expression of YBX1 and the expression of other genes related to ER status. The results indicated that ER status is associated with an increased expression of YBX1 and that YBX1 was strongly associated with ESR1 and other genes that enhance ER-independent growth.

Knockdown of YBX1 caused a concomitant drop in gene and protein expression. Knockdown of CORO1C did not alter the expression of YB-1, however, it did result in significant reductions in cell migration and invasion. Thus, siRNA knockdown of YBX1 inhibits tumor growth. YBX1 is a novel biomarker for breast cancer.

The serum of patients suffering from breast cancer is enriched with YBX1 (YB-1) protein that has multiple functions. It is a cold-shock protein. It has been linked to many cancer-related hallmarks like tumor growth, invasion , and metastasis. Different cell types release YB-1 , and it can act as an extracellular mitogen. The study found that the levels of YB-1 in serum were associated with metastatic BC characteristics, including the presence bone metastases.

Research on YBX1 indicate that it is an RNA binding protein that is able to be secreted to cancer cells. These studies also proved that the knockdown of YBX1 reduced AC073352.1 expression in pancreatic cancer cells. This suggests that YBX1 may assist in loading AC073352.1 into excosomes. Further, the protein is involved in tumor metastasis.

It is tested in a wide range of samples

The YBX1 marker is a biosensor which detects the hNTh2 protein in a variety of biological samples. The hNTh2 protein interacts with YB1, and studies have demonstrated that high levels nuclear YB1 are associated with drug resistance and poor outcomes for patients. The Boster Bio YBX1 Marker is verified in a variety samples.

The Boster Bio-YBX1 Markers have been validated in a range of biological specimens such as tumor cells and human milk. The YB-1 protein, a part of the cold-shock protein family, is a typical example of how cells control gene expression. It associates with mRNA in the cytoplasm. It also controls translation. In cancer, high cellular levels of YB-1 have been linked with poor clinical outcomes. Monitoring and detection of YB-1 might help in determining the best treatments for patients with cancer.

Many cancers, including breast cancer, have YBX1 as a marker. However, the use of this marker is limited because of the lack of an appropriate antibody for the detection of the YB-1 receptor in these cancers. A monoclonal antibody to YB-1 would allow for a greater variety of treatment options, a more risk-based profile, and chemotherapy sensitivity. Unfortunately, the polyclonal antibodies detect that YB-1 is only found in the cytoplasm and the activities of YB-1 are believed to occur in the nuclear compartment.

The Boster Bio YBX1 Markers are tested in a variety tissues and fluids. The hNTh2 marker demonstrates the expression of hNTh2 in a variety of samples that include urine, serum, blood, and other biological fluids. Additionally, the hNTh2 mTQ2 protein is expressed in MCF7 cells.

It is an antibody monoclonal

The prototypical cold shock protein member. It orchestrates transcription of proliferation-related genes, associates with mRNA and directs translation within the cytoplasm. In cancer, cellular YB-1 expression is associated with poor outcomes in clinical trials. Therefore, YB-1 detection and measurement may be a useful tool to aid patients in determining which treatment regimen is best for them.

A high-affinitymonoclonal antigen for YBX1 is available from Boster Bio. The monoclonal antibody tested against recombinant proteins human tissues, as well as untransfected cells. This results in high sensitivity and affinity. The antibody was also tested against 250 different tissues.

Prospective studies that seek to verify the effectiveness of YB-1 as a breast cancer marker can employ a monoclonal antibody which was recently designed to detect nuclear YB-1. It could also be used to adjust chemotherapy regimens for patients with breast cancer. The researchers acknowledge the help of Ellen Krott, who performed immunohistochemistry. The study was supported by grants from the Deutsche Forschungsgemeinschaft (DFG). In addition, PRM, a founder of antibodies-online, has filed a patent for its monoclonal anti-E2G5 antibody.

The YBX1 marker is tested in WB and IHC-P applications and can be purchased from Boster Bio under catalog number A01054S102. It has been tested for the detection of YB-1 in Human, Mouse, Rat and Mouse. The antibody is not effective in YBX1 immunoreactive tumour cells and may not be useful for certain diagnostic tests.

It can be utilized in a myriad of tests

The YBX1 marker can be used to detect protein-protein interactions. It is affordable and highly specific. The YBX1 marker is versatile and can be used in a variety tests. The hNTh2-YBX1 interface has been identified as a key druggable target. As such, the biosensor can be a highly efficient screening tool.

This antibody has been tested in IP, IF, and IHC. It was confirmed by binding to YBX1 in several assays. It is able to react with a variety of species including Rats, Humans and Mouse. YBX1 has been used in various tests in the lab, including tests to diagnose cancer, predict the prognosis of patients, and measure the sensitivity to chemotherapy.

Human fibroblast cells were used to isolate the YBX1 gene. The gene sequences were amplified using PCR and then ligated to pGEX-6P-1 vectors. The YBX1 translocated gene was expressed in transfected cells using pGEX-6P-1. Western Blot analysis was carried out by using a monoclonal antibody against the YBX1.

Boster Bio YBX1 is a gene which is frequently expressed in breast cancer. It is linked to poor overall survival rates , as well as distant metastasis. It has also been associated with the development of inflammation-related diseases. BRD7 protein has also been linked with the expression of the YBX1 gene. This protein is linked to cancer progression and poor survival, so an increase in YB1 expression could indicate the risk of the invasiveness.

It is available through Boster Bio

The YBX1 marker is a product of Boster Bio. Boster Bio manufactures the YBX1 marker. It is made from E. coli and contains an His-Tag that makes it suitable for use in SDS-PAGE. For a period of one week, YBX1 proteins can be stored at 2-8 degrees Celsius. It can also be stored between -20 and -80 degrees Celsius. Repetition of freeze-thai cycles is not recommended. Scientists from all over the world can use this YBX1 marker for research.