Anti-XRN1 Antibody Picoband™

Product Name

Anti-XRN1 Antibody Picoband™

View all Xrn1 Antibodies

SKU/Catalog Number

A02855-2

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-XRN1 Antibody Picoband™ catalog # A02855-2. Tested in WB, ICC/IF, ELISA applications. This antibody reacts with Human, Mouse.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-XRN1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02855-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Immunogen

E.coli-derived human XRN1 recombinant protein (Position: K523-H1198). Human XRN1 shares 93.2% amino acid (aa) sequence identity with mouse XRN1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A02855-2 is reactive to Xrn1 in Human, Mouse

Applications

A02855-2 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

200 kDa

Calculated molecular weight

Background of Xrn1

5′-3′ exoribonuclease 1 (Xrn1) is a protein that in humans is encoded by the XRN1 gene. This gene encodes a member of the 5'-3' exonuclease family. The encoded protein may be involved in replication-dependent histone mRNA degradation, and interacts directly with the enhancer of mRNA-decapping protein 4. In addition to mRNA metabolism, a similar protein in yeast has been implicated in a variety of nuclear and cytoplasmic functions, including homologous recombination, meiosis, telomere maintenance, and microtubule assembly. Mutations in this gene are associated with osteosarcoma, suggesting that the encoded protein may also play a role in bone formation. Alternative splicing results in multiple transcript variants.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human, Mouse
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of XRN1 using anti-XRN1 antibody (A02855-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human SiHa whole cell lysates,
Lane 3: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-XRN1 antigen affinity purified polyclonal antibody (Catalog # A02855-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for XRN1 at approximately 200 kDa. The expected band size for XRN1 is at 194 kDa.

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Figure 2. IF analysis of XRN1 using anti-XRN1 antibody (A02855-2).
XRN1 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-XRN1 Antibody (A02855-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Gene/Protein Information For Xrn1 (Source: Uniprot.org, NCBI)

Alternative Names

5'-3' exoribonuclease 1; DKFZp434P0721; DKFZp686B22225; DKFZp686F19113; EC 3.1.11.-; SEP1FLJ41903; Strand-exchange protein 1 homolog Xrn1|Dhm2, exo, mXr, mXrn1|5-3 exoribonuclease 1|5-3 exoribonuclease 1|protein Dhm2|strand-exchange protein 1 homolog

For more info on Xrn1, check out the Xrn1 Infographic

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In this infographic, you will see the following information for Xrn1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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