DNA repair protein XRCC3 Antibodies

DNA repair protein XRCC3 (XRCC3)

Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. Part of the RAD21 paralog protein complex CX3 which acts from the BRCA1-BRCA2- dependent HR pathway.

Upon DNA damage, CX3 acts downstream of RAD51 recruitment; the complex binds predominantly to the junction of the four duplex arms of the Holliday junction (HJ) and to junctions of replication forks. Involved in HJ resolution and thus in processing HR intermediates late in the DNA repair process; the function may be linked to the CX3 complex and seems to involve GEN1 during mitotic cell cycle progression.

Gene Information

Human
Gene Name:	XRCC3
Uniprot:	O43542
Entrez:	7517

Family

Belongs to:
RecA family

Alternative Names

CMM6; DNA repair protein XRCC3; X-ray repair complementing defective repair in Chinese hamster cells 3; X-ray repair cross-complementing protein 3

Molecular Weight

Mass (kDA):
37.85 kDA

Genomic Context

Human
Location:	14q32.33
Sequence:	14; NC_000014.9 (103697611..103715486, complement)

Subcellular Localizations

Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Mitochondrion. Accumulates in discrete nuclear foci prior to DNA damage, and these foci persist throughout the time course of DNA repair.

Diseases

• Malignant Neoplasms
• Neoplasms
• Malignant Neoplasm Of Breast
• Mammary Neoplasms
• Malignant Neoplasm Of Lung
• Carcinoma
• Lung Neoplasms
• Malignant Squamous Cell Neoplasm
• Cytogenetic Abnormality
• Adenocarcinoma
• Malignant Neoplasm Of Urinary Bladder
• Colorectal Neoplasms
• Carcinogenesis

• Non-small Cell Lung Carcinoma
• Bladder Neoplasm
• Colorectal Cancer
• Xeroderma
• Xeroderma Pigmentosum
• Head And Neck Neoplasms
• Malignant Head And Neck Neoplasm

Interacting Proteins

• PALB2
• RAD51
• RAD51C
• SWSAP1

Pathways

• Dna Repair
• Double-strand Break Repair
• Cell Cycle
• Recombinational Repair
• Mismatch Repair
• Pathogenesis
• Hypersensitivity
• Dna Replication
• Meiosis
• Gene Conversion
• S Phase
• Localization
• Mitosis

• Cell Proliferation
• Telomere Maintenance
• Single Strand Break Repair
• Dna Recombination
• Chromosome Segregation
• Sensitization
• Menarche

PTMs

• Phosphorylation
• Methylation

• Acetylation
• Oxidation

Research Areas

• Apoptosis
• Cancer
• DNA Repair
• Genes Sensitive to DNA Damaging Agents
• Homologous Recombination

• Tumor Suppressors

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/XRCC3

Best Uses Of The XRCC3 Marker In Breast Cancer

The XRCC3 X-ray crystal protein is a marker of gene entry in multiple sexes. It is expressed as an E. coli protein and has a his-tag for SDS/PAGE/denatured applications. This marker can be stored at +2degC to (+8degC) for up to one week. It is stable in -20 to +80degC. It reacts well with multiple species and can be used for a variety of purposes.

XRCC3Marker

We used RT-PCR to test the efficacy of the XRCC3 marker for predicting DSS in breast carcinoma. TRIZOL extracted total RNA from ESCC cell lines. cDNA was synthesized with MMLV reverse transcriptase. We first determined the expression level of the XRCC3 gene in ESCC cells. The expression data were then normalized against GAPDH. To analyze the expression level of the XRCC3 gene, we used PCR with primers for the XRCC3 gene and the RAD51C marker.

The XRCC3 genetic polymorphism rs1799794 can be associated with various types of cancer. However the specific associations between this variant and increased cancer risk are not clear. To explore whether this polymorphism contributes to cancer risk, we performed a meta-analysis of studies involving this gene. We searched PubMed, Embase and the Cochrane Library to find relevant studies from June 11, 2019. Stata 14.0 was also used for subgroup analyses. Subgroups were identified based on type of cancer, ethnicity, source control, detection method, and source.

Methylation in candidate genes is associated with low immune checkpoint expression in some types of cancers. This association can be causal in histological desegregation. In inflammatory lung carcinoma, XRCC3 methylation has been associated with decreased expression of E-Cadherin or Vimentin. Similar associations were observed in cervical and lung tumors. Further studies are required to determine the role of methylation in cancer.

XRCC3 protein

The XRCC3 protein is a member of the RecA family and plays an important role in DNA homologous recombination (HR). It is needed for the repair or translocation of chromosomal chromosomes and other genetic defects. This protein interacts with Rad51 protein to maintain chromosomal integrity. It also contributes to the prevention of mutations and helps repair DNA damage resulting from carcinogens.

The XRCC3 protein plays a critical role in DNA repair. Although it is only found in very small quantities in the brain, it enhances the process by interfacing with the human Rad51C Protein, which has the highest sequence identity. There are many important differences among the two proteins. In particular, Xrcc3Rad51C displays a lower elution profile than Rad51C.

XRCC3Rad51C binds single stranded DNA (dsDNA). It does NOT contain intramolecular base pair, unlike Rad51C. When Rad51C and Xrcc3Rad51C can bind single-stranded RNA, they create a filamentous structure which promotes homologous pair. Homologous pairing is possible only if XRCC3Rad51C and Rad51C are able to bind single-stranded DNA. Rad51C activity drops if there is no Xrcc3.

XRCC3 is a protein with a complex of other proteins called RAD51C. Rad51C belongs to this group. Xrcc3 interacts in vitro with both of these proteins and forms a stable interacting complex. It isn't clear which of the two proteins plays RAD51C. This may explain the different roles these proteins play in our bodies.

Rad51C coexpresses in E. coli with XRCC3. To express the proteins, the Rad51C cell line was used. After coexpression, cells expressing both proteins were produced in a recAJM109(DE3) s strain. The proteins were identified using Affi–Gel-heparin column analysis and Coomassie Brilliant Blue staining.

XRCC2 knockdown improves radiotherapy sensitivity of colon cancer cells. The in vitro and in vivo radiotherapy response to colon tumor cells is enhanced by knockdowns of XRCC2. XRCC2 knockdown might be a therapeutic target for colon carcinoma. Boster Bio provides more information on the research. Once clinical trials are approved, XRCC3 inhibiters will be available for the medical community.

XRCC3Rad51C promotes homologous pairage between single-stranded and double-stranded genes. It is not required to be superhelical for this activity. XRCC3Rad51C promotes insertion of the double helix into homologous regions in dsDNA. XRCC3Rad51C promotes homologous matching when paired up with pGsat4DNA.

Yan et.al. have recently conducted a metaanalysis of studies. Yan et.al. analyzed all literature on XRCC3 Thr241Met polymorphism within a Caucasian community. The meta-analysis comprised seven studies. These studies revealed a weak link between XRCC3 polymorphisms and leukemia risk. However, further studies are necessary to determine the role of other confirmed genetic and environmental factors.

XRCC1+399A/G - SNP

The XRCC3 XRCC3 Gene, which encodes a Protein, is expressed within E. coli. The protein is suitable to be used in SDS-PAGE and other denatured applications. The antibody is stable in two temperatures. They can be kept at +2degC or +8degC one week, and then stored at -20degC until eight months. It reacts with proteins of different species, and can be stored at two to eight degrees Celsius.