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Product Info Summary
| SKU: | PB9964 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-WNK1 Antibody Picoband®
SKU/Catalog Number
PB9964
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-WNK1 Antibody Picoband® catalog # PB9964. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-WNK1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9964)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human WNK1, different from the related mouse and rat sequences by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9964 is reactive to WNK1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
250 kDa
Calculated molecular weight
250794 MW
Background of WNK1
WNK lysine deficient protein kinase 1, also known as WNK1, is an enzyme that in humans is encoded by the WNK1 gene. The human gene is located on short arm of chromosome 12 (12p13.3). This gene encodes a member of the WNK subfamily of serine/threonine protein kinases. The encoded protein may be a key regulator of blood pressure by controlling the transport of sodium and chloride ions. Mutations in this gene have been associated with pseudohypoaldosteronism type II and hereditary sensory neuropathy type II. Alternatively spliced transcript variants encoding different isoforms have been described but the full-length nature of all of them has yet to be determined.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9964 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Rat, Human
Positive Control
WB: rat testis tissue
IHC: mouse testis tissue, rat testis tissue, human intestinal cancer tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of WNK1 using anti-WNK1 antibody (PB9964).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat testis tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WNK1 antigen affinity purified polyclonal antibody (Catalog # PB9964) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WNK1 at approximately 250 kDa. The expected band size for WNK1 is at 251 kDa.
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Figure 2. IHC analysis of WNK1 using anti-WNK1 antibody (PB9964).
WNK1 was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-WNK1 Antibody (PB9964) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 3. IHC analysis of WNK1 using anti-WNK1 antibody (PB9964).
WNK1 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-WNK1 Antibody (PB9964) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of WNK1 using anti-WNK1 antibody (PB9964).
WNK1 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-WNK1 Antibody (PB9964) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For WNK1 (Source: Uniprot.org, NCBI)
Gene Name
WNK1
Full Name
Serine/threonine-protein kinase WNK1
Weight
250794 MW
Superfamily
protein kinase superfamily
Alternative Names
Serine/threonine-protein kinase WNK1;2.7.11.1;Erythrocyte 65 kDa protein;p65;Kinase deficient protein;Protein kinase lysine-deficient 1;Protein kinase with no lysine 1;hWNK1;WNK1;HSN2, KDP, KIAA0344, PRKWNK1; WNK1 HSAN2, HSN2, KDP, PPP1R167, PRKWNK1, PSK, p65 WNK lysine deficient protein kinase 1 serine/threonine-protein kinase WNK1|erythrocyte 65 kDa protein|prostate-derived sterile 20-like kinase|protein kinase with no lysine 1|protein phosphatase 1, regulatory subunit 167|serine/threonine-protein kinase WNK1 1|serine/threonine-protein kinase WNK1 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on WNK1, check out the WNK1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for WNK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-WNK1 Antibody Picoband® (PB9964)
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Customer Q&As
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7 Customer Q&As for Anti-WNK1 Antibody Picoband®
Question
What is the formulation for product PB9964?
Verified customer
Asked: 2021-02-23
Answer
For the Anti-WNK1 Antibody Picoband™ (PB9964), each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Boster Scientific Support
Answered: 2021-02-24
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for neuron using anti-WNK1 antibody PB9964. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-02-25
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-25
Question
Would anti-WNK1 antibody PB9964 work for IHC with neuron?
Verified Customer
Verified customer
Asked: 2020-01-07
Answer
According to the expression profile of neuron, WNK1 is highly expressed in neuron. So, it is likely that anti-WNK1 antibody PB9964 will work for IHC with neuron.
Boster Scientific Support
Answered: 2020-01-07
Question
Would PB9964 anti-WNK1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-10-22
Answer
You can see on the product datasheet, PB9964 anti-WNK1 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-10-22
Question
Is this PB9964 anti-WNK1 antibody reactive to the isotypes of WNK1?
B. Baker
Verified customer
Asked: 2017-04-10
Answer
The immunogen of PB9964 anti-WNK1 antibody is A synthetic peptide corresponding to a sequence in the middle region of human WNK1 (1154-1195aa DNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSED), different from the related mouse and rat sequences by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-04-10
Question
I see that the anti-WNK1 antibody PB9964 works with IHC, what is the protocol used to produce the result images on the product page?
A. Edwards
Verified customer
Asked: 2016-01-11
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2016-01-11
Question
We are currently using anti-WNK1 antibody PB9964 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on feline tissues as well?
N. Krishna
Verified customer
Asked: 2013-08-08
Answer
The anti-WNK1 antibody (PB9964) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-08-08
