Product Info Summary
SKU: | M01910 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-WDR5 Monoclonal Antibody
SKU/Catalog Number
M01910
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-WDR5 Monoclonal Antibody catalog # M01910. Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-WDR5 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01910)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ADHA-23
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human WDR5
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01910 is reactive to WDR5 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
50 kDa
Calculated molecular weight
43296 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01910 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
FC 1:50
Validation Images & Assay Conditions
Click image to see more details
All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
Click image to see more details
Western blot analysis of WDR5 expression in HeLa cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For WDR5 (Source: Uniprot.org, NCBI)
Gene Name
WDR5
Full Name
WD repeat-containing protein 5
Weight
43296 MW
Superfamily
WD repeat WDR5/wds family
Alternative Names
Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial;1.2.4.1;PDHE1-A type I;PDHA1;PHE1A; WDR5 BIG-3, CFAP89, SWD3 WD repeat domain 5 WD repeat-containing protein 5|BMP2-induced 3-kb gene protein|SWD3, Set1c WD40 repeat protein, homolog|cilia and flagella associated protein 89
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on WDR5, check out the WDR5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for WDR5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-WDR5 Monoclonal Antibody (M01910)
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No publications found for M01910
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Customer Q&As
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4 Customer Q&As for Anti-WDR5 Monoclonal Antibody
Question
What sample was tested in WB and IHC applications with M01910?
Verified customer
Asked: 2020-01-09
Answer
Western blot analysis of WDR5 expression in HeLa cell lysate.
Boster Scientific Support
Answered: 2020-01-09
Question
We are currently using anti-WDR5 Monoclonal antibody M01910 for human tissue, and we are satisfied with the Flow Cytometry results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-23
Answer
The anti-WDR5 Monoclonal antibody (M01910) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-23
Question
I was wanting to use your anti-WDR5 Monoclonal antibody for WB for mouse testis on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse testis identification?
Verified Customer
Verified customer
Asked: 2019-08-14
Answer
It shows on the product datasheet, M01910 anti-WDR5 Monoclonal antibody has been validated for Flow Cytometry, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-14
Question
My question regarding product M01910, anti-WDR5 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
P. Zhang
Verified customer
Asked: 2013-03-04
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01910 anti-WDR5 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-03-04