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Product Info Summary
| SKU: | A12216 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-WDR24 Antibody Picoband®
SKU/Catalog Number
A12216
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-WDR24 Antibody Picoband® catalog # A12216. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-WDR24 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12216)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human WDR24 recombinant protein (Position: H309-H762).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A12216 is reactive to WDR24 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
88 kDa
Calculated molecular weight
88.207kDa
Background of WDR24
WD repeat-containing protein 24 is a protein that in humans is encoded by the WDR24 gene. WDR24 is a component ofthe GATOR2 complex which hasfive known subunits(WDR24, WDR59, Mios, Sec13, and Seh1L). GATOR2 complex positively regulates mTORC1 signaling by acting upstream of orin parallel to GATOR1, which is a GTPase-activating protein (GAP) for RagA or RagB and an inhibitor ofthe amino-acid-sensing pathway.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A12216 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human 293T whole cell, human placenta tissue, monkey COS-7 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of WDR24 using anti-WDR24 antibody (A12216).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human placenta tissue lysates,
Lane 3: monkey COS-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WDR24 antigen affinity purified polyclonal antibody (Catalog # A12216) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WDR24 at approximately 88 kDa. The expected band size for WDR24 is at 88 kDa.
Protein Target Info & Infographic
Gene/Protein Information For WDR24 (Source: Uniprot.org, NCBI)
Gene Name
WDR24
Full Name
GATOR complex protein WDR24
Weight
88.207kDa
Superfamily
WD repeat WDR24 family
Alternative Names
Mitochondrial import inner membrane translocase subunit Tim17-A; Inner membrane preprotein translocase Tim17a; TIMM17A; MIMT17; TIM17; TIM17A; TIMM17 WDR24 C16orf21, JFP7 WD repeat domain 24 GATOR complex protein WDR24|WD repeat-containing protein 24
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on WDR24, check out the WDR24 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for WDR24: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-WDR24 Antibody Picoband® (A12216)
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No publications found for A12216
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