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Product Info Summary
| SKU: | M05438 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-WASL Monoclonal Antibody
SKU/Catalog Number
M05438
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-WASL Monoclonal Antibody catalog # M05438. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-WASL Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05438)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ACOB-23
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human WASL Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex. Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M05438 is reactive to WASL in Human, Mouse
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
55 kDa
Calculated molecular weight
50327 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05438 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50
Positive Control
WB: HeLa cell
Validation Images & Assay Conditions
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Western blot analysis of WASL expression in HeLa cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For WASL (Source: Uniprot.org, NCBI)
Gene Name
WASL
Full Name
Neural Wiskott-Aldrich syndrome protein
Weight
50327 MW
Alternative Names
Tubulin beta-1 chain;TUBB1; WASL N-WASP, NWASP, WASPB WASP like actin nucleation promoting factor neural Wiskott-Aldrich syndrome protein|Wiskott-Aldrich syndrome like
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on WASL, check out the WASL Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for WASL: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-WASL Monoclonal Antibody (M05438)
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No publications found for M05438
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Customer Q&As
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6 Customer Q&As for Anti-WASL Monoclonal Antibody
Question
I see that the anti-WASL Monoclonal antibody M05438 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-11-19
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-11-19
Question
Would M05438 anti-WASL Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-10-22
Answer
As indicated on the product datasheet, M05438 anti-WASL Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-22
Question
Can you help my question with product M05438, anti-WASL Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-09-27
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M05438 anti-WASL Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-27
Question
I was wanting to use your anti-WASL Monoclonal antibody for WB for mouse colon on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse colon identification?
Verified Customer
Verified customer
Asked: 2019-08-02
Answer
As indicated on the product datasheet, M05438 anti-WASL Monoclonal antibody has been validated for IF, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse colon in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-02
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for colon using anti-WASL Monoclonal antibody M05438. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-05-15
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-15
Question
We are currently using anti-WASL Monoclonal antibody M05438 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2017-06-23
Answer
The anti-WASL Monoclonal antibody (M05438) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-06-23
