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Product Info Summary
| SKU: | PA1575 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Fascin/FSCN1 Antibody Picoband®
SKU/Catalog Number
PA1575
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Fascin/FSCN1 Antibody catalog # PA1575. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Fascin/FSCN1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1575)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Fascin, identical to the related rat and mouse sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1575 is reactive to FSCN1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
55 kDa
Calculated molecular weight
54530 MW
Background of Fascin
Fascin is a actin cross-linking protein. The Fascin gene contains 5 exons and spans 7 kb. It is a 54-58 kilodalton monomeric actin filament bundling protein originally isolated from sea urchin egg but also found in Drosophila and vertebrates, including humans. Fascin (from the Latin for bundle) is spaced at 11 nanometre intervals along the filament. The bundles in cross section are seen to be hexagonally packed, and the longitudinal spacing is compatible with a model where fascin cross-links at alternating 4 and 5 actins. It is calcium insensitive and monomeric. Fascin binds beta-catenin, and colocalizes with it at the leading edges and borders of epithelial and endothelial cells. The role of Fascin in regulating cytoskeletal structures for the maintenance of cell adhesion, coordinating motility and invasion through interactions with signalling pathways is an active area of research especially from the cancer biology perspective. Abnormal fascin expression or function has been implicated in breast cancer, colon cancer, esophageal squamous cell carcinoma, gallbladder cancer and prostate cancer.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1575 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Positive Control
WB: U87 Whole Cell, A549 Whole Cell, MCF-7 Whole Cell, HT1080 Whole Cell
Validation Images & Assay Conditions
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Anti-Fascin antibody, PA1575, Western blotting
All lanes: Anti Fascin (PA1575) at 0.5ug/ml
Lane 1: U87 Whole Cell Lysate at 40ug
Lane 2: A549 Whole Cell Lysate at 40ug
Lane 3: MCF-7 Whole Cell Lysate at 40ug
Lane 4: HT1080 Whole Cell Lysate at 40ug
Predicted bind size: 55KD
Observed bind size: 55KD
Protein Target Info & Infographic
Gene/Protein Information For FSCN1 (Source: Uniprot.org, NCBI)
Gene Name
FSCN1
Full Name
Fascin
Weight
54530 MW
Superfamily
fascin family
Alternative Names
Fascin;55 kDa actin-bundling protein;Singed-like protein;p55;FSCN1;FAN1, HSN, SNL; FSCN1 FAN1, HSN, SNL, p55 fascin actin-bundling protein 1 fascin|55 kDa actin-bundling protein|epididymis secretory sperm binding protein|fascin homolog 1, actin-bundling protein|singed-like (fascin homolog, sea urchin)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FSCN1, check out the FSCN1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FSCN1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Fascin/FSCN1 Antibody Picoband® (PA1575)
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Customer Q&As
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4 Customer Q&As for Anti-Fascin/FSCN1 Antibody Picoband®
Question
We were well pleased with the WB result of your anti-Fascin/FSCN1 antibody. However we have observed positive staining in cervix carcinoma cytosol using this antibody. Is that expected? Could you tell me where is FSCN1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-02-21
Answer
Based on literature, cervix carcinoma does express FSCN1. Generally FSCN1 expresses in cytoplasm, cytosol. Regarding which tissues have FSCN1 expression, here are a few articles citing expression in various tissues:
Brain, Cajal-Retzius cell, and Fetal brain cortex, Pubmed ID: 3525578
Brain, Eye, Lung, and Muscle, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 24275569
Teratocarcinoma, Pubmed ID: 8068206
Testis, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2020-02-21
Question
We are currently using anti-Fascin/FSCN1 antibody PA1575 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-13
Answer
The anti-Fascin/FSCN1 antibody (PA1575) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-13
Question
We have seen staining in rat cervix carcinoma. What should we do? Is anti-Fascin/FSCN1 antibody supposed to stain cervix carcinoma positively?
R. Zhang
Verified customer
Asked: 2018-06-15
Answer
According to literature cervix carcinoma does express FSCN1. According to Uniprot.org, FSCN1 is expressed in epithelium of bronchus, teratocarcinoma, testis, brain, eye, lung muscle, ovarian carcinoma, brain, cajal-retzius cell fetal brain cortex, cervix carcinoma, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have FSCN1 expression, here are a few articles citing expression in various tissues:
Brain, Cajal-Retzius cell, and Fetal brain cortex, Pubmed ID: 3525578
Brain, Eye, Lung, and Muscle, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 24275569
Teratocarcinoma, Pubmed ID: 8068206
Testis, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2018-06-15
Question
Our lab want to know about using your anti-Fascin/FSCN1 antibody for cell motility studies. Has this antibody been tested with western blotting on a549 whole cell lysate? We would like to see some validation images before ordering.
S. Johnson
Verified customer
Asked: 2017-04-19
Answer
Thanks for your inquiry. This PA1575 anti-Fascin/FSCN1 antibody is tested on u87 whole cell lysate, a549 whole cell lysate, ht1080 whole cell lysate. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-04-19
