Product Info Summary
SKU: | M06407 |
---|---|
Size: | 100 µl |
Reactive Species: | Dog, Human, Monkey, Mouse, Rat |
Host: | Mouse |
Application: | IF, IHC, WB |
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Product info
Product Name
Anti-VPS28 Mouse Monoclonal Antibody [Clone ID: OTI1A8]
SKU/Catalog Number
M06407
Size
100 µl
Description
Boster Bio VPS28 mouse monoclonal antibody, clone OTI1A8 (formerly 1A8). Catalog# M06407. Tested in IF, IHC, WB. This antibody reacts with Human, Monkey, Mouse, Rat, Dog.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-VPS28 Mouse Monoclonal Antibody [Clone ID: OTI1A8] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06407)
Host
Mouse
Contents
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI1A8
Isotype
IgG1
Immunogen
Full length human recombinant protein of human VPS28 (NP_057292) produced in HEK293T cell.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M06407 is reactive to VPS28 in Dog, Human, Monkey, Mouse, Rat
Reconstitution
Observed Molecular Weight
25 kDa
Calculated molecular weight
25.425kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M06407 is guaranteed for IF, IHC, WB Boster Guarantee
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VPS28 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VPS28.
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Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-VPS28 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-VPS28 monoclonal antibody (1:200).
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-VPS28 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Anti-VPS28 mouse monoclonal antibody (M06407) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY VPS28.
Protein Target Info & Infographic
Gene/Protein Information For VPS28 (Source: Uniprot.org, NCBI)
Gene Name
VPS28
Full Name
Vacuolar protein sorting-associated protein 28 homolog
Weight
25.425kDa
Superfamily
VPS28 family
Alternative Names
ESCRT-I complex subunit VPS28; H-Vps28; MGC60323; vacuolar protein sorting 28 (yeast); vacuolar protein sorting 28 homolog (S. cerevisiae); vacuolar protein sorting-associated protein 28 homolog; yeast class E protein Vps28p homolog VPS28 VPS28 subunit of ESCRT-I vacuolar protein sorting-associated protein 28 homolog|ESCRT-I complex subunit VPS28|VPS28, ESCRT-I subunit|vacuolar protein sorting 28 homolog|vacuolar protein sorting 28-like protein|yeast class E protein Vps28p homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on VPS28, check out the VPS28 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for VPS28: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-VPS28 Mouse Monoclonal Antibody [Clone ID: OTI1A8] (M06407)
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