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Product Info Summary
| SKU: | PB10106 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-VNN1 Antibody Picoband®
View all Vanin-1/VNN1 Antibodies
SKU/Catalog Number
PB10106
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-VNN1 Antibody Picoband® catalog # PB10106. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-VNN1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10106)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human VNN1 recombinant protein (Position: Q22-K192). Human VNN1 shares 82.2% amino acid (aa) sequence identity with mouse VNN1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB10106 is reactive to VNN1 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
57 kDa
Calculated molecular weight
57012 MW
Background of Vanin-1/VNN1
Pantetheinase is an enzyme that in humans is encoded by the VNN1 gene. This gene encodes a member of the vanin family of proteins, which share extensive sequence similarity with each other, and also with biotinidase. The family includes secreted and membrane-associated proteins, a few of which have been reported to participate in hematopoietic cell trafficking. No biotinidase activity has been demonstrated for any of the vanin proteins, however, they possess pantetheinase activity, which may play a role in oxidative-stress response. This protein, like its mouse homolog, is likely a GPI-anchored cell surface molecule. The mouse protein is expressed by the perivascular thymic stromal cells and regulates migration of T-cell progenitors to the thymus. This gene lies in close proximity to, and in the same transcriptional orientation as, two other vanin genes on chromosome 6q23-q24.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB10106 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: SKOV3 whole cell, HELA whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of VNN1 using anti-VNN1 antibody (PB10106).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: SKOV3 whole cell lysates,
Lane 2: HELA whole cell lysates lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VNN1 antigen affinity purified polyclonal antibody (Catalog # PB10106) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VNN1 at approximately 57 kDa. The expected band size for VNN1 is at 57 kDa.
Protein Target Info & Infographic
Gene/Protein Information For VNN1 (Source: Uniprot.org, NCBI)
Gene Name
VNN1
Full Name
Pantetheinase
Weight
57012 MW
Superfamily
carbon-nitrogen hydrolase superfamily
Alternative Names
Pantetheinase;3.5.1.92 ;Pantetheine hydrolase;Tiff66;Vascular non-inflammatory molecule 1;Vanin-1;VNN1; VNN1 HDLCQ8, Tiff66 vanin 1 pantetheinase|pantetheine hydrolase|vannin 1|vascular non-inflammatory molecule 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on VNN1, check out the VNN1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for VNN1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-VNN1 Antibody Picoband® (PB10106)
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Customer Q&As
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7 Customer Q&As for Anti-VNN1 Antibody Picoband®
Question
I was wanting to use your anti-VNN1 antibody for WB for human plasma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human plasma identification?
Verified Customer
Verified customer
Asked: 2020-01-08
Answer
As indicated on the product datasheet, PB10106 anti-VNN1 antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human plasma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-08
Question
Does PB10106 anti-VNN1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-07-02
Answer
As indicated on the product datasheet, PB10106 anti-VNN1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-07-02
Question
Is this PB10106 anti-VNN1 antibody reactive to the isotypes of VNN1?
Verified Customer
Verified customer
Asked: 2017-08-09
Answer
The immunogen of PB10106 anti-VNN1 antibody is E. coli-derived human VNN1 recombinant protein (Position: Q22-K192). Human VNN1 shares 82.2% amino acid (aa) sequence identity with mouse VNN1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-08-09
Question
I have a question about product PB10106, anti-VNN1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
M. Parker
Verified customer
Asked: 2017-05-03
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB10106 anti-VNN1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-05-03
Question
Do you have a BSA free version of anti-VNN1 antibody PB10106 available?
B. Johnson
Verified customer
Asked: 2016-02-02
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-VNN1 antibody PB10106 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-02-02
Question
Does anti-VNN1 antibody PB10106 work on feline WB with bile?
G. Krishna
Verified customer
Asked: 2014-05-02
Answer
Our lab technicians have not tested anti-VNN1 antibody PB10106 on feline. You can run a BLAST between feline and the immunogen sequence of anti-VNN1 antibody PB10106 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline bile in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-05-02
Question
We are currently using anti-VNN1 antibody PB10106 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on bovine tissues as well?
L. Collins
Verified customer
Asked: 2013-06-04
Answer
The anti-VNN1 antibody (PB10106) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-06-04
