Product Info Summary
SKU: | EZ1312 |
---|---|
Size: | For 5 plates, 96 wells each |
Reactive Species: | Human |
Application: | ELISA |
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Product info
Product Name
Human CD32/FCGR2b/c ELISA Kit EZ-Set™ (DIY Antibody Pairs)
SKU/Catalog Number
EZ1312
Size
For 5 plates, 96 wells each
*Question: How many samples can I assay/run in this kit?
Description
Human CD32/FCGR2b/c ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human FCGR2B in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CD32/FCGR2b/c ELISA Kit EZ-Set™ (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ1312)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: A46-P217
Assay Range
46.9 pg/ml - 3,000 pg/ml
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EZ1312 is reactive to FCGR2B in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EZ1312 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
Catalog number | Description | Quantity | Storage of opened/reconstituted material |
---|---|---|---|
EZ1312-CA | Rabbit anti- human FCGR2B/C polyclonal antibody (Capture Antibody) | 500 µl, 0.4 mg/mL (recommended dilution 1:100) | Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. |
EZ1312-DA | Biotinylated goat anti- human FCGR2B/C polyclonal antibody (Detection Antibody) | 500 µl, 6 µg/mL (recommended dilution 1:100) | |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 500 µl (recommended dilution 1:100) | |
EK1312-ST | Lyophilized recombinant human FCGR2B/C standard | 10 ng/tube×3 | Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- 1. Microplate reader in standard size.
- 2. Automated plate washer.
- 3. Incubator.
- 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- 5. Clean tubes and Eppendorf tubes.
- 6. 96 well microplate (Cat# AR1100)
- 7. Plate Sealers.
- 8. Capture Antibody Diluent: PBS.
- 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
- 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
- 11. Stop Solution: 2 N H2SO4 (Cat# AR1105)
- 12. Wash Buffer (PBS and PBS-T). Recipes:
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4. - *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
Concentration (pg/ml) | 0 | 46.9 | 93.8 | 187.5 | 375 | 750 | 1500 | 3000 |
O.D. | 0.007 | 0.124 | 0.199 | 0.255 | 0.541 | 0.813 | 1.408 | 2.266 |
Data Example Images
Click image to see more details
Human CD32/FCGR2b/c EZ-Set ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml) | 147 | 890 | 1865 | 168 | 904 | 2162 |
Standard deviation | 6.62 | 32.93 | 80.2 | 9.75 | 41.6 | 112.42 |
CV (%) | 4.5% | 3.7% | 4.3% | 5.8% | 4.6% | 5.2% |
Protein Target Info & Infographic
Gene/Protein Information For FCGR2B (Source: Uniprot.Org, NCBI)
Gene Name
FCGR2B
Full Name
Low affinity immunoglobulin gamma Fc region receptor II-b
Weight
34.044kDa
Alternative Names
Low affinity immunoglobulin gamma Fc region receptor II-b; IgG Fc receptor II-b; CDw32; Fc-gamma RII-b; Fc-gamma-RIIb; FcRII-b; CD32; FCGR2B; CD32, FCG2, IGFR2 FCGR2B CD32, CD32B, FCG2, FCGR2, FCGR2C, FcRII-c, IGFR2 Fc fragment of IgG receptor IIb low affinity immunoglobulin gamma Fc region receptor II-b|CDw32|Fc fragment of IgG, low affinity II, receptor for (CD32)|Fc fragment of IgG, low affinity IIb, receptor (CD32)|Fc fragment of IgG, low affinity IIb, receptor for (CD32)|Fc gamma RIIb|Fc gamma receptor IIb|Fc-gamma RII-c|Fc-gamma-RIIc|IgG Fc receptor II-c|Low affinity immunoglobulin gamma Fc region receptor II-c|fc-gamma RII-b|fc-gamma-RIIb|fcRII-b|igG Fc receptor II-b
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on FCGR2B, check out the FCGR2B Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for FCGR2B: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human CD32/FCGR2b/c ELISA Kit EZ-Set™ (DIY Antibody Pairs) (EZ1312)
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