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Product Info Summary
| SKU: | RP1108 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC |
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Product info
Product Name
Anti-VIP peptides VIP Antibody
SKU/Catalog Number
RP1108
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-VIP peptides VIP Antibody catalog # RP1108. Tested in IF, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-VIP peptides VIP Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1108)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human VIP, different from the related mouse and rat sequences by four amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
RP1108 is reactive to VIP in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
26 kDa
Calculated molecular weight
19169 MW
Background of VIP
Vasoactive intestinal peptide, also known as PHM27 or VIP, is a peptide hormone containing 28 amino acid residues. This gene is mapped to 6q25. The protein encoded by this gene belongs to the glucagon family. It stimulates myocardial contractility, causes vasodilation, increases glycogenolysis, lowers arterial blood pressure and relaxes the smooth muscle of trachea, stomach and gall bladder. The protein also acts as an antimicrobial peptide with antibacterial and antifungal activity. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
RP1108 is guaranteed for IF, IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunofluorescence, 5 μg/ml, Rat
Positive Control
IHC: human pancreatic cancer tissue, rat pancreas tissue, rat intestine tissue, mouse pancreas tissue, mouse intestine tissue
IF: rat colon tissue
Validation Images & Assay Conditions
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Figure 1. IHC analysis of VIP using anti-VIP antibody (RP1108).
VIP was detected in a paraffin-embedded section of human pancreatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-VIP Antibody (RP1108) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 2. IHC analysis of VIP using anti-VIP antibody (RP1108).
VIP was detected in a paraffin-embedded section of rat pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-VIP Antibody (RP1108) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of VIP using anti-VIP antibody (RP1108).
VIP was detected in a paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-VIP Antibody (RP1108) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of VIP using anti-VIP antibody (RP1108).
VIP was detected in a paraffin-embedded section of mouse pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-VIP Antibody (RP1108) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 5. IHC analysis of VIP using anti-VIP antibody (RP1108).
VIP was detected in a paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-VIP Antibody (RP1108) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 6. IF analysis of VIP using anti-VIP antibody (RP1108).
VIP was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-VIP Antibody (RP1108) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For VIP (Source: Uniprot.org, NCBI)
Gene Name
VIP
Full Name
VIP peptides
Weight
19169 MW
Superfamily
glucagon family
Alternative Names
VIP peptides;Intestinal peptide PHV-42;Peptide histidine valine 42;Intestinal peptide PHM-27;Peptide histidine methioninamide 27;Vasoactive intestinal peptide;VIP;Vasoactive intestinal polypeptide;VIP; VIP PHM27 vasoactive intestinal peptide VIP peptides|prepro-VIP
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on VIP, check out the VIP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for VIP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-VIP peptides VIP Antibody (RP1108)
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Customer Q&As
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16 Customer Q&As for Anti-VIP peptides VIP Antibody
Question
Our lab were content with the WB result of your anti-VIP antibody. However we have seen positive staining in pancreatic carcinoma secreted. using this antibody. Is that expected? Could you tell me where is VIP supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-05-01
Answer
Based on literature, pancreatic carcinoma does express VIP. Generally VIP expresses in secreted. Regarding which tissues have VIP expression, here are a few articles citing expression in various tissues:
Pancreatic carcinoma, Pubmed ID: 6139527
Pheochromocytoma, Pubmed ID: 1318039
Prostate, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-05-01
Question
My question regarding product RP1108, anti-VIP antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-28
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free RP1108 anti-VIP antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-28
Question
I see that the anti-VIP antibody RP1108 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-01-13
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-01-13
Question
I was wanting to use your anti-VIP antibody for IHC for rat fundus of stomach on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat fundus of stomach identification?
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
It shows on the product datasheet, RP1108 anti-VIP antibody has been validated for IHC on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat fundus of stomach in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-13
Question
What are the suggestion concentrations for IHC and IF applications?
Verified Customer
Verified customer
Asked: 2019-12-06
Answer
The suggested concentration for immunohistochemistry is 1ug/mL and for immuno fluorescence is 1-2ug/mL
Boster Scientific Support
Answered: 2019-12-06
Question
I would like to test anti-VIP antibody RP1108 on rat fundus of stomach for research purposes, then I may be interested in using anti-VIP antibody RP1108 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
A. Moore
Verified customer
Asked: 2019-12-05
Answer
The products we sell, including anti-VIP antibody RP1108, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-12-05
Question
We have seen staining in human fundus of stomach. Are there any suggestions? Is anti-VIP antibody supposed to stain fundus of stomach positively?
Verified Customer
Verified customer
Asked: 2019-12-02
Answer
From literature fundus of stomach does express VIP. From Uniprot.org, VIP is expressed in fundus of stomach, prostate, pancreatic carcinoma, pheochromocytoma, among other tissues. Regarding which tissues have VIP expression, here are a few articles citing expression in various tissues:
Pancreatic carcinoma, Pubmed ID: 6139527
Pheochromocytoma, Pubmed ID: 1318039
Prostate, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-12-02
Question
Does anti-VIP antibody RP1108 work for IHC with fundus of stomach?
Verified Customer
Verified customer
Asked: 2019-10-18
Answer
According to the expression profile of fundus of stomach, VIP is highly expressed in fundus of stomach. So, it is likely that anti-VIP antibody RP1108 will work for IHC with fundus of stomach.
Boster Scientific Support
Answered: 2019-10-18
Question
Is a blocking peptide available for product anti-VIP antibody (RP1108)?
Verified Customer
Verified customer
Asked: 2019-07-24
Answer
We do provide the blocking peptide for product anti-VIP antibody (RP1108). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-07-24
Question
Do you have a BSA free version of anti-VIP antibody RP1108 available?
Verified Customer
Verified customer
Asked: 2019-06-14
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-VIP antibody RP1108 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-06-14
Question
We are currently using anti-VIP antibody RP1108 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-24
Answer
The anti-VIP antibody (RP1108) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-24
Question
Anti-VIP Antibody RP1108 I am interested in VIP antibody and I wonder compatibility this Antibody for ELISA assay? Has the antibody been tested for ELISA application?
Verified Customer
Verified customer
Asked: 2018-12-14
Answer
RP1108 has not been tested for ELISA application. According to the immunogen sequence, it will not likely to work for ELISA.
Boster Scientific Support
Answered: 2018-12-14
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for fundus of stomach using anti-VIP antibody RP1108. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-10-01
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-10-01
Question
Is this RP1108 anti-VIP antibody reactive to the isotypes of VIP?
Verified Customer
Verified customer
Asked: 2018-09-04
Answer
The immunogen of RP1108 anti-VIP antibody is A synthetic peptide corresponding to a sequence in the middle region of human VIP (81-107aa HADGVFTSDFSKLLGQLSAKKYLESLM), different from the related mouse and rat sequences by four amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-09-04
Question
Would RP1108 anti-VIP antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-07-11
Answer
You can see on the product datasheet, RP1108 anti-VIP antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-07-11
Question
See below the WB image, lot number and protocol we used for fundus of stomach using anti-VIP antibody RP1108. Please let me know if you require anything else.
G. Li
Verified customer
Asked: 2018-01-02
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-01-02
