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Product Info Summary
| SKU: | A01207-1-Biotin |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Chicken, Human, Mouse, Rabbit, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Vinculin VCL Antibody (biotin)
SKU/Catalog Number
A01207-1-Biotin
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Vinculin VCL Antibody (biotin) (Catalog # A01207-1-Biotin). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat, Rabbit, Chicken.
Storage & Handling
Biotin-Vinculin antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-Vinculin VCL Antibody (biotin) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01207-1-Biotin)
Host
Rabbit
Contents
Biotin-Vinculin Antibody is supplied in PBS containing 1% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Biotin-Vinculin antibody was raised against a 23 amino acid peptide near the amino terminus of human Vinculin.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Biotin-Vinculin antibody is human, mouse, rat, rabbit and chicken reactive. At least three isoforms of Vinculin are known to exist.
Reactive Species
A01207-1-Biotin is reactive to VCL in Chicken, Human, Mouse, Rabbit, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
123799 MW
Background of Vinculin
Vinculin is a cytoskeletal protein that plays an important role in the regulation of focal adhesions and embryonic development (1). Three structural vinculin domains include an amino-terminal head, a short flexible proline-rich region and a carboxy-terminal tail (2). Expression of vinculin were shown to be affected by the level of actin expression (2,3). Vinculin deficiencies are associated with a decrease in cell adhesion and an increase in cell motility, suggesting a possible role in metastatic growth (4). Defects in VCL are the cause of cardiomyopathy dilated type 1W (CMD1W) (5).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01207-1-Biotin is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Vinculin antibody can be used for detection of Vinculin by Western blot at 0.5 - 1 μg/ml.
Antibody validated: Western Blot in human, mouse, and rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of Vinculin in 293, A431, A549, Daudi, HeLa, HepG2, K562, 3T3, Raji, U937 cell lysate and mouse brain, rat brain, rabbit brain, mouse lung, rat lung, mouse liver, rat liver, rabbit spleen, chicken liver and chicken small intestine tissue lysate with Biotin-Vinculin antibody at 1 μg/ml.
Protein Target Info & Infographic
Gene/Protein Information For VCL (Source: Uniprot.org, NCBI)
Gene Name
VCL
Full Name
Vinculin
Weight
123799 MW
Superfamily
vinculin/alpha-catenin family
Alternative Names
CMD1W, CMH15, Metavinculin, MVCL VCL CMD1W, CMH15, HEL114, MV, MVCL vinculin vinculin|epididymis luminal protein 114|epididymis secretory sperm binding protein|meta-vinculin|metavinculin|vinculin isoform meta-VCL
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on VCL, check out the VCL Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for VCL: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Vinculin VCL Antibody (biotin) (A01207-1-Biotin)
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