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Product Info Summary
| SKU: | M04065-3 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-CD10 (MME) Mouse Monoclonal Antibody [Clone ID: OTI3D11]
View all Neprilysin/CD10 Antibodies
SKU/Catalog Number
M04065-3
Size
100 µl
Description
Boster Bio MME (CD10) mouse monoclonal antibody, clone OTI3D11 (formerly 3D11). Catalog# M04065-3. Tested in FC, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD10 (MME) Mouse Monoclonal Antibody [Clone ID: OTI3D11] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04065-3)
Host
Mouse
Contents
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI3D11
Isotype
IgG2a
Immunogen
Full length human recombinant protein of human MME (NP_009218) produced in HEK293T cell.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M04065-3 is reactive to MME in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
85.3 kDa
Calculated molecular weight
85.514kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04065-3 is guaranteed for Flow Cytometry, WB Boster Guarantee
Validation Images & Assay Conditions
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Flow cytometric analysis of living 293T cells transfected with MME overexpression plasmid
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-MME mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-MME mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MME mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MME mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MME mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-MME mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-MME mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-MME mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-MME mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MME (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MME (1:500).
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Western blot analysis of extracts (35ug) from 1 tissue lysate by using anti-MME monoclonal antibody (1:500).
Protein Target Info & Infographic
Gene/Protein Information For MME (Source: Uniprot.org, NCBI)
Gene Name
MME
Full Name
Neprilysin
Weight
85.514kDa
Superfamily
peptidase M13 family
Alternative Names
Atriopeptidase; CALLA; CALLAmembrane metallo-endopeptidase (neutral endopeptidase, enkephalinase); CD10 antigen; CD10; CD10); CD10membrane metallo-endopeptidase variant 1; Common acute lymphocytic leukemia antigen; DKFZp686O16152; EC 3.4.24; EC 3.4.24.11; Enkephalinase; EPN; Leu-19; membrane metallo-endopeptidase variant 2; membrane metallo-endopeptidase; MGC126681; MGC126707; MME; NEPmembrane metallo-endopeptidase (neutral endopeptidase, enkephalinase, CALLA; Neprilysin; Neutral Endopeptidase 24.11; Neutral endopeptidase; NKH1; SFE; Skin fibroblast elastase MME CALLA, CD10, CMT2T, NEP, SCA43, SFE membrane metalloendopeptidase neprilysin|atriopeptidase|common acute lymphocytic leukemia |membrane metallo-endopeptidase (neutral endopeptidase, enkephalinase, CALLA, CD10)|membrane metallo-endopeptidase variant 1|membrane metallo-endopeptidase variant 2|neprilysin-390|neprilysin-411|neutral endopeptidase 24.11|skin fibroblast elastase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MME, check out the MME Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MME: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD10 (MME) Mouse Monoclonal Antibody [Clone ID: OTI3D11] (M04065-3)
Hello CJ!
M04065-3 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zhang C,Chen Y,Sun S,Zhang Y,Wang L,Luo Z,Liu M,Dong L,Dong N,Wu Q. A conserved LDL-receptor motif regulates corin and CD320 membrane targeting in polarized renal epithelial cells. Elife.2020 Nov 2;9:e56059. doi:10.7554/eLife.56059.PMID:33136001;PMCID:PMC
Species: Human
M04065-3 usage in article: APP:IHC, SAMPLE:KIDNEY TISSUES, DILUTION:1:100
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