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Product Info Summary
| SKU: | M04205 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Villin (GI-Mucosal & Urogenital Brush Border Marker) VIL1 Monoclonal Antibody
SKU/Catalog Number
M04205
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-Villin (GI-Mucosal & Urogenital Brush Border Marker) VIL1 Monoclonal Antibody (Catalog # M04205). Tested in WB, IHC applications. This antibody reacts with Human.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-Villin (GI-Mucosal & Urogenital Brush Border Marker) VIL1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04205)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: VIL1/1325
Isotype
IgG1, kappa
Immunogen
Recombinant human Villin fragment (around aa179-311) (exact sequence is proprietary))
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M04205 is reactive to VIL1 in Human
Reconstitution
Reconstitute with distilled water.
Observed Molecular Weight
68 kDa
Calculated molecular weight
92695 MW
Background of Villin 1
Recognizes a protein of 95kDa, which is identified as villin. It is a major constituent in the microvilli, which compose the brush border of epithelial cells forming absorptive surfaces of the intestinal and renal proximal tubular epithelia. Anti-Villin labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract. Among neoplasms, villin is predominantly expressed in tumors of colorectal origin. Antibody to villin is useful in identifying malignant cells from primary and metastatic colorectal carcinomas. This antibody also labels Merkel cells of the skin.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04205 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot (1-2ug/ml for 60 minutes at RT)
Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
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Formalin-fixed, paraffin-embedded human Rectum stained with Anti-Villin Mouse Monoclonal Antibody (VIL1/1325).
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Formalin-fixed, paraffin-embedded human Colon stained with Anti-Villin Mouse Monoclonal Antibody (VIL1/1325).
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Western Blot of A549, HepG2 & HCT116 cell lysates using Anti-Villin Mouse Monoclonal Antibody (VIL1/1325).
Protein Target Info & Infographic
Gene/Protein Information For VIL1 (Source: Uniprot.org, NCBI)
Gene Name
VIL1
Full Name
Villin-1
Weight
92695 MW
Superfamily
villin/gelsolin family
Alternative Names
Villin-1;VIL1;VIL; VIL1 D2S1471, VIL villin 1 villin-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on VIL1, check out the VIL1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for VIL1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Villin (GI-Mucosal & Urogenital Brush Border Marker) VIL1 Monoclonal Antibody (M04205)
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No publications found for M04205
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Customer Q&As
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4 Customer Q&As for Anti-Villin (GI-Mucosal & Urogenital Brush Border Marker) VIL1 Monoclonal Antibody
Question
Does anti-Villin (GI-Mucosal & Urogenital Brush Border Marker) Monoclonal antibody M04205 work on primate IHC with intestine?
Verified Customer
Verified customer
Asked: 2020-03-20
Answer
Our lab technicians have not validated anti-Villin (GI-Mucosal & Urogenital Brush Border Marker) Monoclonal antibody M04205 on primate. You can run a BLAST between primate and the immunogen sequence of anti-Villin (GI-Mucosal & Urogenital Brush Border Marker) Monoclonal antibody M04205 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate intestine in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-20
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for kidney using anti-Villin (GI-Mucosal & Urogenital Brush Border Marker) Monoclonal antibody M04205. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-08-12
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-12
Question
Would anti-Villin (GI-Mucosal & Urogenital Brush Border Marker) Monoclonal antibody M04205 work for IHC with kidney?
V. Parker
Verified customer
Asked: 2017-12-22
Answer
According to the expression profile of kidney, VIL1 is highly expressed in kidney. So, it is likely that anti-Villin (GI-Mucosal & Urogenital Brush Border Marker) Monoclonal antibody M04205 will work for IHC with kidney.
Boster Scientific Support
Answered: 2017-12-22
Question
I see that the anti-Villin (GI-Mucosal & Urogenital Brush Border Marker) Monoclonal antibody M04205 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-10-30
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-10-30
