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Product Info Summary
| SKU: | A00079-2 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC-P, WB |
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Product info
Product Name
Anti-VHL Antibody (C-term)
View all Von Hippel Lindau Antibodies
SKU/Catalog Number
A00079-2
Size
80 µl
Form
Liquid
Description
Boster Bio Anti-VHL Antibody (C-term) (Catalog # A00079-2). Tested in IHC-P, Flow Cytometry, WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-VHL Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00079-2)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This VHL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 181-210 amino acids from the C-terminal region of human VHL.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00079-2 is reactive to VHL in Human
Reconstitution
Calculated molecular weight
24153 Da
Background of Von Hippel Lindau
Von Hippel-Lindau syndrome (VHL) is a dominantly inherited familial cancer syndrome predisposing to a variety of malignant and benign tumors. A germline mutation of VHL gene is the basis of familial inheritance of VHL syndrome. The protein is a component of the protein complex that includes elongin B, elongin C, and cullin-2, and possesses ubiquitin ligase E3 activity. This protein is involved in the ubiquitination and degradation of hypoxia-inducible-factor (HIF), which is a transcription factor that plays a central role in the regulation of gene expression by oxygen. RNA polymerase II subunit POLR2G/RPB7 is also reported to be a target of this protein.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00079-2 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:50-1:100
FC: 1:10-1:50
Validation Images & Assay Conditions
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Anti-VHL Antibody (C-term) at 1:1000 dilution + Raji whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 24 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Western blot analysis of VHL antibody (C-term) in HepG2 cell line lysates (35ug/lane). VHL (arrow) was detected using the purified Pab.
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VHL Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the VHL Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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VHL Antibody (C-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Protein Target Info & Infographic
Gene/Protein Information For VHL (Source: Uniprot.org, NCBI)
Gene Name
VHL
Full Name
von Hippel-Lindau disease tumor suppressor
Weight
24153 Da
Superfamily
VHL family
Alternative Names
Von Hippel-Lindau disease tumor suppressor, Protein G7, pVHL, VHL VHL HRCA1, RCA11, pVHL, VHL von Hippel-Lindau tumor suppressor von Hippel-Lindau disease tumor suppressor|elongin binding protein|protein G7|von Hippel-Lindau tumor suppressor, E3 ubiquitin protein ligase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on VHL, check out the VHL Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for VHL: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-VHL Antibody (C-term) (A00079-2)
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