Product Info Summary
SKU: | A00045-8 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Mouse |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-Vegfa Antibody Picoband™
SKU/Catalog Number
A00045-8
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Vegfa Antibody Picoband™ catalog # A00045-8. Tested in WB, ELISA applications. This antibody reacts with Mouse.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Vegfa Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00045-8)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived mouse Vegfa recombinant protein (Position: A205-R392). Mouse Vegfa shares 89.4% and 98.4% amino acid (aa) sequence identity with human and rat Vegfa, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00045-8 is reactive to VEGFA in Mouse
Applications
A00045-8 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
26 kDa
Calculated molecular weight
Background of VEGFA
VEGF, a homodimeric glycoprotein of relative molecular mass 45,000, is the only mitogen that specifically acts on endothelial cells. It may be a major regulator of tumor angiogenesis in vivo. Vascular endothelial growth factor is a mitogen primarily for vascular endothelial cells. It is, however, structurally related to platelet-derived growth factor. VEGF shares homology with the PDGF A chain and B chain, including conservation of all 8 cysteines found in PDGFA and PDGFB. VEGF gene contains 8 exons. Vascular endothelial growth factor(VEGF) induces remodeling and enhances TH2-mediated sensitization and inflammation in the lung. VEGF regulates haematopoietic stem cell survival by an internal autocrine loop mechanism. Vascular endothelial growth factor(VEGF) stimulates neurogenesis in vitro and in vivo.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse
ELISA, 0.1-0.5 μg/ml, Mouse
Validation Images & Assay Conditions
![a00045 8 vegfa primary antibodies wb testing 1 a00045 8 vegfa primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a00045-8-vegfa-primary-antibodies-wb-testing-1.jpg)
Click image to see more details
Figure 1. Western blot analysis of Vegfa using anti-Vegfa antibody (A00045-8).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Vegfa antigen affinity purified polyclonal antibody (Catalog # A00045-8) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Vegfa at approximately 26 kDa. The expected band size for Vegfa is at 43 kDa.
Protein Target Info & Infographic
Gene/Protein Information For VEGFA (Source: Uniprot.org, NCBI)
Gene Name
VEGFA
Full Name
Vascular endothelial growth factor A
Weight
Superfamily
PDGF/VEGF growth factor family
Alternative Names
MVCD1; VAS; vascular endothelial growth factor A; Vascular permeability factor; Vasculotropin; VEGF; VEGFA; VEGF-A; VEGFMGC70609; VPF; VPFvascular endothelial growth factor VEGFA MVCD1, VEGF, VPF vascular endothelial growth factor A vascular endothelial growth factor A|vascular endothelial growth factor A121|vascular endothelial growth factor A165|vascular permeability factor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on VEGFA, check out the VEGFA Infographic
![VEGFA infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for VEGFA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Vegfa Antibody Picoband™ (A00045-8)
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