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Product Info Summary
| SKU: | EK0541 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate). |
Product info
Product Name
Mouse VEGF ELISA Kit PicoKine®
SKU/Catalog Number
EK0541
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse VEGF ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Vegfa in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse VEGF ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0541)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: A27-R190
Sensitivity
<2 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0541 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0541 is reactive to Vegfa in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Application Guarantee
EK0541 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of VEGFA
Vascular permeability factor/vascular endothelial growth factor (VPF/VEGF), a potent cytokine expressed by most malignant tumors, has critical roles in vasculogenesis and both physiological and pathological angiogenesis. VEGF produced by tumor cells potently stimulates endothelial cell proliferation and angiogenesis and plays a key role in the pathophysiology of several neoplasias. VEGF may also play a pivotal role in mediating the development and progression of diabetic retinopathy. VEGF, a major regulator of angiogenesis, binds to two receptor tyrosine kinases, KDR/Flk-1 and Flt-1.The VEGF gene is mapped by fluorescence in situ hybridization to chromosome 6p12. The standard product used in this kit is recombinant mouse VEGF164, a 42KDa dimer.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0541-CAP | Anti-Mouse Vegfa Pre-coated 96-well strip microplate | 1 |
| EK0541-ST | Mouse Vegfa Standard | 2 vials, 10 ng/tube |
| EK0541-DA | Mouse Vegfa Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.035 | 0.074 | 0.131 | 0.211 | 0.353 | 0.660 | 1.201 | 1.936 |
Data Example Images
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Mouse VEGF PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect VEGFA, Dilution ratio of 1:1, concentration in serum and plasma is less than the lowest standard, 15.6 pg/ml..
Some articles we found to cite concentrations of VEGFA in samples: 21451264 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse VEGF ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 40 | 179 | 538 | 40 | 171 | 574 |
| Standard deviation | 1.92 | 9.48 | 36.58 | 2.6 | 9.57 | 48.79 |
| CV (%) | 4.8% | 5.3% | 6.8% | 6.5% | 5.6% | 8.5% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of VEGFA in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 40 | 34 | 35 | 37 | 36 | 2.29 | 6.3% |
| Sample 2 | 179 | 204 | 186 | 197 | 191 | 9.65 | 5% |
| Sample 3 | 538 | 463 | 494 | 471 | 491 | 29.15 | 5.9% |
Protein Target Info & Infographic
Gene/Protein Information For Vegfa (Source: Uniprot.Org, NCBI)
Gene Name
Vegfa
Full Name
Vascular endothelial growth factor A
Weight
25283 MW
Superfamily
PDGF/VEGF growth factor family
Alternative Names
Vascular endothelial growth factor A;VEGF-A;Vascular permeability factor;VPF;Vegfa;Vegf; VEGFA MVCD1, VEGF, VPF vascular endothelial growth factor A vascular endothelial growth factor A|vascular endothelial growth factor A121|vascular endothelial growth factor A165|vascular permeability factor
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Vegfa, check out the Vegfa Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Vegfa: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse VEGF ELISA Kit PicoKine® (EK0541)
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Customer Q&As
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6 Customer Q&As for Mouse VEGF ELISA Kit PicoKine®
Question
Have other isoforms been tested for cross-reactivity with EK0541 and EK0539?
Verified customer
Asked: 2019-09-25
Answer
The The Human VEGF ELISA Kit PicoKine EK0539 and Mouse VEGF ELISA Kit PicoKine EK0541 are cross-reactive with their specific targets only.
Boster Scientific Support
Answered: 2019-09-26
Question
Q: how to thaw whole blood sample for VEGFA ELISA after freezing?
Verified Customer
Verified customer
Asked: 2018-04-07
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test VEGFA for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2018-04-07
Question
Q: can I use EDTA plasma as samples in Mouse VEGFA Picokine® ELISA Kit (Catalog # EK0541)?
Verified Customer
Verified customer
Asked: 2018-01-27
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of VEGFA causing degradation of its protein structure. VEGFA may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the VEGFA ELISA, treating samples with different anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-01-27
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2017-07-06
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2017-07-06
Question
Are the mouse, rat, and human VEGF kits truly specific for each species or do they cross react? Which isoforms of VEGF do the human, mouse and rat kit detect? Keyword: specificity
Verified Customer
Verified customer
Asked: 2017-06-08
Answer
Our VEGF Elisa kits are truly specific for each species, and they detect 165 amino acid form.
Boster Scientific Support
Answered: 2017-06-08
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring VEGFA using the EK0541 Mouse VEGFA Picokine® ELISA Kit?
D. Banerjee
Verified customer
Asked: 2017-03-26
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to avoid protein degradation.
Boster Scientific Support
Answered: 2017-03-26
