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Product Info Summary
| SKU: | M01372 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-VAPB Mouse Monoclonal Antibody [Clone ID: OTI4D8]
SKU/Catalog Number
M01372
Size
100 µl
Description
Boster Bio VAPB mouse monoclonal antibody, clone OTI4D8 (formerly 4D8). Catalog# M01372. Tested in WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-VAPB Mouse Monoclonal Antibody [Clone ID: OTI4D8] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01372)
Host
Mouse
Contents
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI4D8
Isotype
IgG1
Immunogen
Human recombinant protein fragment corresponding to amino acids 2-223 of human VAPB (NP_004729) produced in E.coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01372 is reactive to VAPB in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
27 kDa
Calculated molecular weight
27.228kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01372 is guaranteed for WB Boster Guarantee
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of VAPB using anti-VAPB antibody (M01372).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human COLO320 whole cell lysates,
Lane 4: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-VAPB antigen affinity purified monoclonal antibody (Catalog # M01372) at at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for VAPB at approximately 27 kDa. The expected band size for VAPB is at 27 kDa.
Protein Target Info & Infographic
Gene/Protein Information For VAPB (Source: Uniprot.org, NCBI)
Gene Name
VAPB
Full Name
Vesicle-associated membrane protein-associated protein B/C
Weight
27.228kDa
Superfamily
VAMP-associated protein (VAP) (TC 9.B.17) family
Alternative Names
ALS8; ALS8VAMP-B/VAMP-C; VAMP (vesicle-associated membrane protein)-associated protein B and C; VAMP-associated 33 kDa protein; VAMP-associated protein B/C; VAMP-B; VAMP-C; VAPB; VAP-B; VAP-B/VAP-C; VAPC; VAP-C; vesicle-associated membrane protein-associated protein B/C VAPB ALS8, VAMP-B, VAP-B VAMP associated protein B and C vesicle-associated membrane protein-associated protein B/C|VAMP (vesicle-associated membrane protein)-associated protein B and C|VAMP-associated 33 kDa protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on VAPB, check out the VAPB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for VAPB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-VAPB Mouse Monoclonal Antibody [Clone ID: OTI4D8] (M01372)
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