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Product Info Summary
| SKU: | A10546 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Alpha Defensin 1/DEFA1 Antibody Picoband®
View all alpha-Defensin 1 Antibodies
SKU/Catalog Number
A10546
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Alpha Defensin 1/DEFA1 Antibody Picoband® catalog # A10546. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Alpha Defensin 1/DEFA1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10546)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Alpha Defensin 1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A10546 is reactive to DEFA1 in Human, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
19 kDa
Calculated molecular weight
10201 MW
Background of alpha-Defensin 1
Defensin, alpha 1, also known as human alpha defensin 1, human neutrophil peptide 1 (HNP-1) or neutrophil defensin 1 is a human protein that is encoded by the DEFA1 gene. Defensins are a family of antimicrobial and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. The protein encoded by this gene, defensin, alpha 1, is found in the microbicidal granules of neutrophils and likely plays a role in phagocyte-mediated host defense. Several alpha defensin genes are clustered on chromosome 8. This gene differs from defensin, alpha 3 by only one amino acid. This gene and the gene encoding defensin, alpha 3 are both subject to copy number variation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10546 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat
Positive Control
WB: rat testis tissue, HELA whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Alpha Defensin 1 using anti-Alpha Defensin 1 antibody (A10546).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
lane 1: rat testis tissue lysate,
lane 2: HELA whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Alpha Defensin 1 antigen affinity purified polyclonal antibody (Catalog # A10546) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Alpha Defensin 1 at approximately 19KD. The expected band size for Alpha Defensin 1 is at 10KD.
Protein Target Info & Infographic
Gene/Protein Information For DEFA1 (Source: Uniprot.org, NCBI)
Gene Name
DEFA1
Full Name
Neutrophil defensin 1
Weight
10201 MW
Superfamily
alpha-defensin family
Alternative Names
Neutrophil defensin 1;Defensin, alpha 1;HNP-1;HP-1;HP1;HP 1-56;Neutrophil defensin 2;HNP-2;HP-2;HP2;DEFA1;DEF1, DEFA2, MRS;DEFA1B; DEFA1 DEF1, DEFA2, HNP-1, HP-1, HP1, MRS defensin alpha 1 neutrophil defensin 1|defensin, alpha 1, myeloid-related sequence|defensin, alpha 2|myeloid-related sequence
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DEFA1, check out the DEFA1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DEFA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Alpha Defensin 1/DEFA1 Antibody Picoband® (A10546)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-Alpha Defensin 1/DEFA1 Antibody Picoband®
Question
Will anti-Alpha Defensin 1/DEFA1 antibody A10546 work on zebrafish WB with lung?
R. Bhatt
Verified customer
Asked: 2020-05-08
Answer
Our lab technicians have not validated anti-Alpha Defensin 1/DEFA1 antibody A10546 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-Alpha Defensin 1/DEFA1 antibody A10546 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish lung in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-05-08
Question
Is a blocking peptide available for product anti-Alpha Defensin 1/DEFA1 antibody (A10546)?
Verified Customer
Verified customer
Asked: 2020-05-05
Answer
We do provide the blocking peptide for product anti-Alpha Defensin 1/DEFA1 antibody (A10546). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-05-05
Question
Does anti-Alpha Defensin 1/DEFA1 antibody A10546 work for WB with lung?
Verified Customer
Verified customer
Asked: 2020-01-16
Answer
According to the expression profile of lung, DEFA1 is highly expressed in lung. So, it is likely that anti-Alpha Defensin 1/DEFA1 antibody A10546 will work for WB with lung.
Boster Scientific Support
Answered: 2020-01-16
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-Alpha Defensin 1/DEFA1 antibody A10546. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-31
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-31
Question
We are currently using anti-Alpha Defensin 1/DEFA1 antibody A10546 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, rat. Is it true that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-30
Answer
The anti-Alpha Defensin 1/DEFA1 antibody (A10546) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-30
Question
Does A10546 anti-Alpha Defensin 1/DEFA1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-07-25
Answer
As indicated on the product datasheet, A10546 anti-Alpha Defensin 1/DEFA1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-25
Question
I was wanting to use your anti-Alpha Defensin 1/DEFA1 antibody for WB for human lung on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human lung identification?
A. Moore
Verified customer
Asked: 2013-09-30
Answer
As indicated on the product datasheet, A10546 anti-Alpha Defensin 1/DEFA1 antibody has been tested for WB on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in human lung in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-09-30
