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Product Info Summary
| SKU: | A02331 |
|---|---|
| Size: | 200μg/vial |
| Reactive Species: | Bovine, Guinea pig, Human, Mouse, Rabbit, Rat |
| Host: | Rabbit |
| Application: | IP, ICC, WB |
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Product info
Product Name
Anti-VAMP2 Antibody
SKU/Catalog Number
A02331
Size
200μg/vial
Form
Liquid
Description
Boster Bio Anti-VAMP2 Antibody (Catalog# A02331). Tested in ICC, IP, WB application(s). This antibody reacts with Human, Mouse, Rat, Bovine, Guinea pig, Rabbit.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-VAMP2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02331)
Host
Rabbit
Contents
Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic peptide corresponding to a portion of rat VAMP2. The sequence is completely conserved in mouse, human, bovine, and Xenopus
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A02331 is reactive to Vamp2 in Bovine, Guinea pig, Human, Mouse, Rabbit, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
12691 MW
Background of VAMP-2
SOD3 (SUPEROXIDE DISMUTASE 3) also called SUPEROXIDE DISMUTASE, EXTRACELLULAR, EC-SOD, and Cu-Zn, is an enzyme that in humans is encoded by the SOD3 gene. This gene encodes a member of the superoxide dismutase (SOD) protein family. SODs are antioxidant enzymes that catalyze the dismutation of two superoxide radicals into hydrogen peroxide and oxygen. Hendrickson et al. (1990) mapped the SOD3 gene to 4pter-q21 by a study of somatic cell hybrids. Stern et al. (2003) narrowed the assignment to 4p15.3-p15.1 by somatic cell and radiation hybrid analysis, linkage mapping, and FISH. The product of this gene is though to protect the brain, lungs, and other tissues from oxidative stress. The protein is secreted into the extracellular space and forms a glycosylated homotetramer that is anchored to the extracellular matrix (ECM) and cell surfaces through an interaction with heparan sulfate proteoglycan and collagen. A fraction of the protein is cleaved near the C-terminus before secretion to generate circulating tetramers that do not interact with the ECM.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02331 is guaranteed for IP, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot (1:1,000, ECL). Detects a band of ~18kDa by Western blot.
Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.
Validation Images & Assay Conditions
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Western blot analysis of VAMP2 expression in MW Marker (lane 1), mouse brain extract (lane 2) and rat brain extract (lane 3). VAMP2 at 18KD was detected using rabbit anti-VAMP2 Antigen Affinity purified polyclonal antibody (Catalog # A02331) at 1:1000. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
Protein Target Info & Infographic
Gene/Protein Information For Vamp2 (Source: Uniprot.org, NCBI)
Gene Name
Vamp2
Full Name
Vesicle-associated membrane protein 2
Weight
12691 MW
Superfamily
synaptobrevin family
Alternative Names
Vesicle-associated membrane protein 2;VAMP-2;Synaptobrevin-2;Vamp2;Syb2; VAMP2 NEDHAHM, SYB2, VAMP-2 vesicle associated membrane protein 2 vesicle-associated membrane protein 2|synaptobrevin 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Vamp2, check out the Vamp2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Vamp2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-VAMP2 Antibody (A02331)
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No publications found for A02331
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