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Product Info Summary
| SKU: | A05711 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Vam6/Vps39-like protein VPS39 Antibody
SKU/Catalog Number
A05711
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Vam6/Vps39-like protein VPS39 Antibody (Catalog # A05711). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
VPS39 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Vam6/Vps39-like protein VPS39 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05711)
Host
Rabbit
Contents
VPS39 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
VPS39 antibody was raised against a 18 amino acid synthetic peptide near the carboxy terminus of human VPS39. The immunogen is located within amino acids 710 - 760 of VPS39.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least four isoforms of VPS39 are known to exist; this antibody will detect all four. VPS39 antibody is predicted not to cross-react with TRAP1.
Reactive Species
A05711 is reactive to VPS39 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
101809 MW
Background of VPS39
VPS39 is the mammalian homolog of the yeast vacuolar protein VAM6, that in mammals, promotes lysosome clustering and fusion in vivo. VPS39 is also thought to modulate the TGF-beta response by coupling the TGF-beta receptor complex to the Smad pathway. Similar to the related protein TRAP1, the VPS39 protein is essential for early embryonic development, with mice lacking VPS39 dying at or before E6.5.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05711 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
VPS39 antibody can be used for detection of VPS39 by Western blot at 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of VPS39 in rat liver tissue lysate with VPS39 antibody at 0.5 μg/mL in (A) the absence and (B) the presence of blocking peptide.
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Immunohistochemistry of VPS39 in mouse liver tissue with VPS39 antibody at 5 μg/mL.
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Immunofluorescence of VPS39 in mouse liver tissue with VPS39 antibody at 20 μg/mL.
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Immunofluorescence of VPS39 in human liver tissue with VPS39 antibody at 5 μg/ml.
Green: VPS39 antibody (A05711)
Red: Phylloidin staining
Blue: DAPI staining
Protein Target Info & Infographic
Gene/Protein Information For VPS39 (Source: Uniprot.org, NCBI)
Gene Name
VPS39
Full Name
Vam6/Vps39-like protein
Weight
101809 MW
Superfamily
VAM6/VPS39 family
Alternative Names
TLP, VAM6, hVam6p, KIAA0770, TLP, TRAP1-like protein VPS39 TLP, VAM6, hVam6p VPS39 subunit of HOPS complex vam6/Vps39-like protein|TRAP1-like protein|VPS39, HOPS complex subunit|vacuolar protein sorting 39 homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on VPS39, check out the VPS39 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for VPS39: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Vam6/Vps39-like protein VPS39 Antibody (A05711)
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