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Product Info Summary
| SKU: | A13619 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB |
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Product info
Product Name
Anti-USP40 Antibody
SKU/Catalog Number
A13619
Size
100μl
Form
Liquid
Description
Boster Bio Anti-USP40 Antibody catalog # A13619. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-USP40 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13619)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human USP40. AA range:1011-1060
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A13619 is reactive to USP40 in Human
Reconstitution
Calculated molecular weight
140130 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A13619 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
Validation Images & Assay Conditions
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Western blot analysis of 293 cells using USP40 Polyclonal Antibody diluted at 1：500. Secondary antibody was diluted at 1:20000
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using USP40 Antibody. The picture on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For USP40 (Source: Uniprot.org, NCBI)
Gene Name
USP40
Full Name
Ubiquitin carboxyl-terminal hydrolase 40
Weight
140130 MW
Superfamily
peptidase C19 family
Alternative Names
Ubiquitin carboxyl-terminal hydrolase 40;3.4.19.12;Deubiquitinating enzyme 40;Ubiquitin thioesterase 40;Ubiquitin-specific-processing protease 40;USP40; USP40 ubiquitin specific peptidase 40 ubiquitin carboxyl-terminal hydrolase 40|deubiquitinating enzyme 40|ubiquitin specific protease 40|ubiquitin thioesterase 40|ubiquitin thiolesterase 40|ubiquitin-specific-processing protease 40
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on USP40, check out the USP40 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for USP40: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-USP40 Antibody (A13619)
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No publications found for A13619
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-USP40 Antibody
Question
Would anti-USP40 antibody A13619 work on primate WB with esophagus teratocarcinoma?
Verified Customer
Verified customer
Asked: 2019-09-25
Answer
Our lab technicians have not validated anti-USP40 antibody A13619 on primate. You can run a BLAST between primate and the immunogen sequence of anti-USP40 antibody A13619 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate esophagus teratocarcinoma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-25
Question
Here is the WB image, lot number and protocol we used for esophagus teratocarcinoma using anti-USP40 antibody A13619. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-08-14
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-14
Question
Does A13619 anti-USP40 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
F. Thomas
Verified customer
Asked: 2018-11-19
Answer
It shows on the product datasheet, A13619 anti-USP40 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-11-19
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for esophagus teratocarcinoma using anti-USP40 antibody A13619. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-06-27
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-06-27
Question
We are currently using anti-USP40 antibody A13619 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on monkey tissues as well?
Z. Yang
Verified customer
Asked: 2015-12-01
Answer
The anti-USP40 antibody (A13619) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-12-01
Question
Is this A13619 anti-USP40 antibody reactive to the isotypes of USP40?
T. Jones
Verified customer
Asked: 2015-06-19
Answer
The immunogen of A13619 anti-USP40 antibody is Synthesized peptide derived from the Internal region of human USP40. at AA rangle: 980-1060. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-06-19
Question
I have a question about product A13619, anti-USP40 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
R. Lewis
Verified customer
Asked: 2014-02-05
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A13619 anti-USP40 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-02-05
