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Product Info Summary
| SKU: | M30823 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-TPRA1 Antibody (N-Term)
SKU/Catalog Number
M30823
Size
50 µl
Description
Boster Bio Anti-TPRA1 Antibody (N-Term) (Catalog # M30823). Tested in WB, Flow Cytometry application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-TPRA1 Antibody (N-Term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M30823)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This TPRA1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 16-47 amino acids from human TPRA1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M30823 is reactive to TPRA1 in Human, Mouse
Reconstitution
Calculated molecular weight
41053 Da
Background of GPR175
Rab GTPase key regulator in vesicle trafficking. Essential for maintaining the integrity of the endosome-trans- Golgi network structure. Together with LRRK2, plays a role in the retrograde trafficking pathway for recycling proteins, such as mannose 6 phosphate receptor (M6PR), between lysosomes and the Golgi apparatus in a retromer-dependent manner. Regulates neuronal process morphology in the intact central nervous system (CNS). May play a role in the formation of typhoid toxin transport intermediates during Salmonella enterica serovar Typhi (S.Typhi) epithelial cell infection.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M30823 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
FC: 1:25
Validation Images & Assay Conditions
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All lanes : Anti-TPRA1 Antibody (N-Term) at 1:2000 dilution
Lane 1: human heart lysate
Lane 2: mouse heart lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 41 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Overlay histogram showing U-2OS cells stained with M30823 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (M30823, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Protein Target Info & Infographic
Gene/Protein Information For TPRA1 (Source: Uniprot.org, NCBI)
Gene Name
TPRA1
Full Name
Transmembrane protein adipocyte-associated 1
Weight
41053 Da
Superfamily
UPF0359 family
Alternative Names
Transmembrane protein adipocyte-associated 1, Integral membrane protein GPR175, Transmembrane protein 227, TPRA1, GPR175, TMEM227
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TPRA1, check out the TPRA1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TPRA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TPRA1 Antibody (N-Term) (M30823)
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