Product Info Summary
SKU: | M05040 |
---|---|
Size: | 100 μl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-USP28 Monoclonal Antibody
SKU/Catalog Number
M05040
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-USP28 Monoclonal Antibody catalog # M05040. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-USP28 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05040)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ADDO-21
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human USP28 Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M05040 is reactive to USP28 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
100 kDa
Calculated molecular weight
57491 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05040 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ICC/IF 1:50-1:200
FC 1:50
Positive Control
Validation Images & Assay Conditions
![m05040 wb m05040 wb](https://www.bosterbio.com/media/catalog/product/m/0/m05040-wb.jpg)
Click image to see more details
Western blot analysis of USP28 expression in A431 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For USP28 (Source: Uniprot.org, NCBI)
Gene Name
USP28
Full Name
Ubiquitin carboxyl-terminal hydrolase 28
Weight
57491 MW
Superfamily
peptidase C19 family
Alternative Names
Deubiquitinating enzyme 28; EC 3.1.2.15; EC 3.4.19.12; KIAA1515ubiquitin carboxyl-terminal hydrolase 28; ubiquitin specific peptidase 28; ubiquitin specific protease 28; ubiquitin thioesterase 28; Ubiquitin thiolesterase 28; Ubiquitin-specific-processing protease 28; USP28 USP28 ubiquitin specific peptidase 28 ubiquitin carboxyl-terminal hydrolase 28|deubiquitinating enzyme 28|ubiquitin carboxyl-terminal hydrolase 28 variant 1|ubiquitin thioesterase 28|ubiquitin thiolesterase 28|ubiquitin-specific-processing protease 28
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on USP28, check out the USP28 Infographic
![USP28 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for USP28: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-USP28 Monoclonal Antibody (M05040)
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No publications found for M05040
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Customer Q&As
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5 Customer Q&As for Anti-USP28 Monoclonal Antibody
Question
I was wanting to use your anti-USP28 Monoclonal antibody for IF for human brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human brain identification?
Verified Customer
Verified customer
Asked: 2020-04-20
Answer
You can see on the product datasheet, M05040 anti-USP28 Monoclonal antibody has been tested for IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-20
Question
Is a blocking peptide available for product anti-USP28 Monoclonal antibody (M05040)?
Z. Krishna
Verified customer
Asked: 2020-02-18
Answer
We do provide the blocking peptide for product anti-USP28 Monoclonal antibody (M05040). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-02-18
Question
We are currently using anti-USP28 Monoclonal antibody M05040 for human tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on goat tissues as well?
J. Brown
Verified customer
Asked: 2018-04-19
Answer
The anti-USP28 Monoclonal antibody (M05040) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-04-19
Question
Would anti-USP28 Monoclonal antibody M05040 work on canine IF with cervix carcinoma erythroleukemia?
K. Patel
Verified customer
Asked: 2018-02-19
Answer
Our lab technicians have not validated anti-USP28 Monoclonal antibody M05040 on canine. You can run a BLAST between canine and the immunogen sequence of anti-USP28 Monoclonal antibody M05040 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine cervix carcinoma erythroleukemia in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-02-19
Question
I see that the anti-USP28 Monoclonal antibody M05040 works with IF, what is the protocol used to produce the result images on the product page?
C. Patel
Verified customer
Asked: 2013-10-11
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2013-10-11