Click image to see more details
Product Info Summary
| SKU: | A02016-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Monkey |
| Host: | Rabbit |
| Application: | ELISA, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-MURF1/TRIM63 Antibody Picoband®
View all MuRF1/TRIM63 Antibodies
SKU/Catalog Number
A02016-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MURF1/TRIM63 Antibody Picoband® catalog # A02016-2. Tested in ELISA, WB applications. This antibody reacts with Human, Monkey. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MURF1/TRIM63 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02016-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human MURF1/TRIM63 recombinant protein (Position: D2-E329).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02016-2 is reactive to TRIM63 in Human, Monkey
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
40 kDa
Calculated molecular weight
37492 MW
Background of MuRF1/TRIM63
E3 ubiquitin-protein ligase TRIM63 is an enzyme that in humans is encoded by the TRIM63 gene. This gene encodes a member of the RING zinc finger protein family found in striated muscle and iris. The product of this gene is an E3 ubiquitin ligase that localizes to the Z-line and M-line lattices of myofibrils. This protein plays an important role in the atrophy of skeletal and cardiac muscle and is required for the degradation of myosin heavy chain proteins, myosin light chain, myosin binding protein, and for muscle-type creatine kinase.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02016-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Monkey
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: monkey heart tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of MURF1/TRIM63 using anti-MURF1/TRIM63 antibody (A02016-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: monkey heart tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MURF1/TRIM63 antigen affinity purified polyclonal antibody (Catalog # A02016-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MURF1/TRIM63 at approximately 40KD. The expected band size for MURF1/TRIM63 is at 40KD.
Protein Target Info & Infographic
Gene/Protein Information For TRIM63 (Source: Uniprot.org, NCBI)
Gene Name
TRIM63
Full Name
E3 ubiquitin-protein ligase TRIM63
Weight
37492 MW
Alternative Names
Tripartite motif-containing protein 6; RING finger protein 89; RING-type E3 ubiquitin transferase TRIM6; TRIM6; RNF89 TRIM63 IRF, MURF1, MURF2, RNF28, SMRZ tripartite motif containing 63 E3 ubiquitin-protein ligase TRIM63|RING-type E3 ubiquitin transferase TRIM63|iris ring finger protein|muscle specific ring finger protein 2|muscle-specific RING finger protein 1|ring finger protein 28|striated muscle RING zinc finger protein|tripartite motif containing 63, E3 ubiquitin protein ligase|tripartite motif-containing protein 63
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TRIM63, check out the TRIM63 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TRIM63: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MURF1/TRIM63 Antibody Picoband® (A02016-2)
Hello CJ!
No publications found for A02016-2
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-MURF1/TRIM63 Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-MURF1/TRIM63 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
