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Product Info Summary
| SKU: | A06182-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-USP25 Antibody Picoband®
SKU/Catalog Number
A06182-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-USP25 Antibody Picoband® catalog # A06182-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-USP25 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06182-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human USP25 recombinant protein (Position: M1-D1053).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A06182-1 is reactive to USP25 in Human, Monkey, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
125 kDa
Calculated molecular weight
49655 MW
Background of USP25
Ubiquitin specific peptidase 25 is a protein that in humans is encoded by the USP25 gene. It is mapped to 21q21.1. Ubiquitin is a highly conserved 76-amino acid protein involved in regulation of intracellular protein breakdown, cell cycle regulation, and stress response. Ubiquitin is released from degraded proteins by disassembly of the polyubiquitin chains, which is mediated by ubiquitin-specific proteases (USPs), such as USP25.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06182-1 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25μg/ml, Human, Mouse, Rat, Monkey
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human HEK293 whole cell, human placenta tissue, monkey liver tissue, monkey kidney tissue, human PC-3 whole cell, human Hela whole cell, human A549 whole cell, rat kidney tissue, rat liver tissue, rat lung tissue, human PC-12 whole cell, mouse lung tissue, mouse RAW2647 whole cell, mouse NIH-3T3 whole cell,
ICC/IF: A549 cell
FCM: HL-60 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of USP25 using anti-USP25 antibody (A06182-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HEK293 whole cell lysates,
Lane 2: human placenta tissue lysates,
Lane 3: monkey liver tissue lysates,
Lane 4: monkey kidney tissue lysates,
Lane 5: human PC-3 whole cell lysates,
Lane 6: human Hela whole cell lysates,
Lane 7: human A549 whole cell lysates,
Lane 8: rat kidney tissue lysates,
Lane 9: rat liver tissue lysates,
Lane 10: rat lung tissue lysates,
Lane 11: human PC-12 whole cell lysates,
Lane 12: mouse lung tissue lysates,
Lane 13: mouse RAW264.7 whole cell lysates,
Lane 14: mouse NIH-3T3 whole cell lysates,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-USP25 antigen affinity purified polyclonal antibody (Catalog # A06182-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for USP25 at approximately 125KD. The expected band size for USP25 is at 125KD.
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Figure 2. IF analysis of USP25 using anti-USP25 antibody (A06182-1).
USP25 was detected in immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-USP25 Antibody (A06182-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 3. Flow Cytometry analysis of HL-60 cells using anti-USP25 antibody (A06182-1).
Overlay histogram showing HL-60 cells stained with A06182-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-USP25 Antibody (A06182-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For USP25 (Source: Uniprot.org, NCBI)
Gene Name
USP25
Full Name
Ubiquitin carboxyl-terminal hydrolase 25
Weight
49655 MW
Superfamily
peptidase C19 family
Alternative Names
Ubiquitin carboxyl-terminal hydrolase 25; Deubiquitinating enzyme 25; USP on chromosome 21; Ubiquitin thioesterase 25; Ubiquitin-specific-processing protease 25; USP25; USP21 USP25 USP21 ubiquitin specific peptidase 25 ubiquitin carboxyl-terminal hydrolase 25|USP on chromosome 21|deubiquitinating enzyme 25|ubiquitin thioesterase 25|ubiquitin thiolesterase 25|ubiquitin-specific processing protease 25
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on USP25, check out the USP25 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for USP25: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-USP25 Antibody Picoband® (A06182-1)
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