Anti-USP22 Antibody Picoband™

Product Name

Anti-USP22 Antibody Picoband™

View all USP22 Antibodies

SKU/Catalog Number

A03887-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-USP22 Antibody Picoband™ catalog # A03887-2. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-USP22 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03887-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human USP22, which shares 89.5% amino acid (aa) sequence identity with mouse USP22.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A03887-2 is reactive to USP22 in Human, Mouse, Rat

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Observed Molecular Weight

68 kDa

Calculated molecular weight

Background of USP22

Ubiquitin specific peptidase 22 is a protein that in humans is encoded by the USP22 gene on chromosome 17. Enables enzyme binding activity; nuclear receptor coactivator activity; and thiol-dependent deubiquitinase. Contributes to H4 histone acetyltransferase activity. Involved in positive regulation of mitotic cell cycle; positive regulation of transcription, DNA-templated; and protein modification by small protein conjugation or removal. Part of SAGA complex.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A03887-2 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat

Positive Control

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of USP22 using anti-USP22 antibody (A03887-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: human CACO-2 whole cell lysates,
Lane 5: human MOLT-4 whole cell lysates,
Lane 6: human SH-SY5Y whole cell lysates,
Lane 7: human U251 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-USP22 antigen affinity purified polyclonal antibody (Catalog # A03887-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for USP22 at approximately 68 kDa. The expected band size for USP22 is at 60 kDa.

Click image to see more details

Figure 2. Western blot analysis of USP22 using anti-USP22 antibody (A03887-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: rat heart tissue lysates,
Lane 3: rat kidney tissue lysates,
Lane 4: rat RH35 whole cell lysates,
Lane 5: mouse liver tissue lysates,
Lane 6: mouse heart tissue lysates,
Lane 7: mouse kidney tissue lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-USP22 antigen affinity purified polyclonal antibody (Catalog # A03887-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for USP22 at approximately 68 kDa. The expected band size for USP22 is at 60 kDa.

Gene/Protein Information For USP22 (Source: Uniprot.org, NCBI)

Alternative Names

Deubiquitinating enzyme 22; EC 3.1.2.15; EC 3.4.19.12; KIAA1063ubiquitin carboxyl-terminal hydrolase 22; KIAA1064; ubiquitin specific peptidase 22; ubiquitin specific peptidase 3-like; ubiquitin specific protease 22; Ubiquitin Thioesterase 22; Ubiquitin thiolesterase 22; ubiquitin-specific processing protease 22; Ubiquitin-specific-processing protease 22; USP22; USP3L USP22 USP3L ubiquitin specific peptidase 22 ubiquitin carboxyl-terminal hydrolase 22|deubiquitinating enzyme 22|ubiquitin specific protease 22|ubiquitin thioesterase 22|ubiquitin thiolesterase 22|ubiquitin-specific processing protease 22

For more info on USP22, check out the USP22 Infographic

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In this infographic, you will see the following information for USP22: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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