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Product Info Summary
| SKU: | M03887 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, ICC, WB |
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Product info
Product Name
Anti-USP22 Monoclonal Antibody
SKU/Catalog Number
M03887
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-USP22 Monoclonal Antibody catalog # M03887. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-USP22 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03887)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ADCG-21
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human USP22 Histone deubiquitinating component of the transcription regulatory histone acetylation (HAT) complex SAGA. Catalyzes the deubiquitination of both histones H2A and H2B, thereby acting as a coactivator.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M03887 is reactive to USP22 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
29 kDa
Calculated molecular weight
20132 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03887 is guaranteed for Flow Cytometry, IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Positive Control
WB: HeLa cell
Validation Images & Assay Conditions
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Western blot analysis of USP22 expression in HeLa cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For USP22 (Source: Uniprot.org, NCBI)
Gene Name
USP22
Full Name
Ubiquitin carboxyl-terminal hydrolase 22
Weight
20132 MW
Superfamily
peptidase C19 family
Alternative Names
Bcl-2-related protein A1;Bcl-2-like protein 5;Bcl2-L-5;Hemopoietic-specific early response protein;Protein BFL-1;Protein GRS;BCL2A1;BCL2L5, BFL1, GRS, HBPA1; USP22 USP3L ubiquitin specific peptidase 22 ubiquitin carboxyl-terminal hydrolase 22|deubiquitinating enzyme 22|ubiquitin specific protease 22|ubiquitin thioesterase 22|ubiquitin thiolesterase 22|ubiquitin-specific processing protease 22
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on USP22, check out the USP22 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for USP22: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-USP22 Monoclonal Antibody (M03887)
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No publications found for M03887
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-USP22 Monoclonal Antibody
Question
Is this M03887 anti-USP22 Monoclonal antibody reactive to the isotypes of USP22?
Verified Customer
Verified customer
Asked: 2019-08-08
Answer
The immunogen of M03887 anti-USP22 Monoclonal antibody is A synthesized peptide derived from human USP22 Histone deubiquitinating component of the transcription regulatory histone acetylation (HAT) complex SAGA. Catalyzes the deubiquitination of both histones H2A and H2B, thereby acting as a coactivator. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-08-08
Question
We are currently using anti-USP22 Monoclonal antibody M03887 for human tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2018-12-21
Answer
The anti-USP22 Monoclonal antibody (M03887) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-12-21
Question
I was wanting to use your anti-USP22 Monoclonal antibody for IF for human uterus on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human uterus identification?
T. Lewis
Verified customer
Asked: 2017-01-04
Answer
As indicated on the product datasheet, M03887 anti-USP22 Monoclonal antibody has been tested for IP, IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human uterus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-01-04
Question
I have a question about product M03887, anti-USP22 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
A. Miller
Verified customer
Asked: 2013-08-05
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M03887 anti-USP22 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-08-05
