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Product Info Summary
| SKU: | M03042 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-USP14 Rabbit Monoclonal Antibody
SKU/Catalog Number
M03042
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-USP14 Rabbit Monoclonal Antibody catalog # M03042. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-USP14 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03042)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ABGI-21
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human USP14
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M03042 is reactive to USP14 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
65 kDa
Calculated molecular weight
56069 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03042 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ICC/IF 1:50-1:200
FC 1:60
Positive Control
WB: HeLa cell, RAW 2647 cell, C6 cell
Validation Images & Assay Conditions
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Western blot analysis of USP14 expression in (1) HeLa cell lysate; (2) RAW 264.7 cell lysate; (3) C6 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For USP14 (Source: Uniprot.org, NCBI)
Gene Name
USP14
Full Name
Ubiquitin carboxyl-terminal hydrolase 14
Weight
56069 MW
Superfamily
peptidase C19 family
Alternative Names
Ubiquitin carboxyl-terminal hydrolase 14;3.4.19.12;Deubiquitinating enzyme 14;Ubiquitin thioesterase 14;Ubiquitin-specific-processing protease 14;USP14;TGT; USP14 TGT ubiquitin specific peptidase 14 ubiquitin carboxyl-terminal hydrolase 14|deubiquitinating enzyme 14|tRNA-guanine transglycosylase, 60-kD subunit|ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase)|ubiquitin specific protease 14 (tRNA-guanine transglycosylase)|ubiquitin thioesterase 14|ubiquitin thiolesterase 14|ubiquitin-specific processing protease 14
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on USP14, check out the USP14 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for USP14: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-USP14 Rabbit Monoclonal Antibody (M03042)
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No publications found for M03042
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Customer Q&As
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3 Customer Q&As for Anti-USP14 Rabbit Monoclonal Antibody
Question
I see that the anti-USP14 Rabbit Monoclonal antibody M03042 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-02-24
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-02-24
Question
Will M03042 anti-USP14 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-12-03
Answer
As indicated on the product datasheet, M03042 anti-USP14 Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-12-03
Question
We are currently using anti-USP14 Rabbit Monoclonal antibody M03042 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?
P. Taylor
Verified customer
Asked: 2015-01-12
Answer
The anti-USP14 Rabbit Monoclonal antibody (M03042) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-01-12
